[Histonet] methanol and wax

From:"Katy Whalley"


A colleague of mine hs been having some trouble with wax embedding/
sectioning her samples. They have been dehydrated through a series of
methanol, rather than ethanol steps, follwed by histoclear and wax as
usual. The samples did not section very well, either not cutting at all
(i.e. the wax cuts leaving a hole) or giving a cracked appearance. She has
been advised that the problem was probably the methanol. Has anyone ever
had this problem, and do you know if there is some way her samples can be
rescued as they are very precious!


Katy Whalley, UCL

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