[Histonet] fixation for frozen tissue
I have several questions about fixing frozen tissue. In the past, we've
always frozen tissue fresh from the animal into frozen embedding media and
then immersing into liquid nitrogen.
I have an investigator that brought frozen tissue that he first fixed in
formalin, then froze. I'm unable to get sections because the tissue is
soft. It seems there is still moisture in the tissue, so I melted the block
& blotted off the tissue, then re-froze. I'm still not able to get sections
although it doesn't seem as mushy now. It still seems like the tissue is
wet.Does anyone have any suggestions?
He was also asking about a protocol for sucrose, and I was wondering if
anyone could get one to me? Also why do people use this procedure, and when
do you need to use it?
Thanks for all your help,
Rita Angel, HT
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