[Histonet] Re: Histonet Digest, Vol 5, Issue 33
You use Santa Cruz Ab's? I've had problems with that Ab.
Try a company called BioCare Medical. (www.biocare.net)
I got their mAb CD31 to work great on Paraffin sections.
They offer a solution for a one step HIER and deparaffinizing.
Lastly, I've moved away from HRP development and now use Biocare's Vulcan
Alkaline Phosphatase. I get beautiful, clean staining of my xenograft
vessels for bright field and, as a bonus, the stain fluoresces hot pink
under a Texas red filter... You get really cool pictures.
Hope this helps!
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