[Histonet] IF on frozen sections
>>Date: Mon, 19 Apr 2004 18:33:35 +0200
>>From: "Gudrun Lang"
>>Subject: [Histonet] IF on frozen sections
>>Content-Type: text/plain; charset="iso-8859-1"
>>A question about immunfluorescence on frozen skin sections:
>>We mount the skin with buffer on the sample-table to freeze it. After
>>sectioning we let the cuts airdry and do the IF (Immunglobulins).
>>Is there any reason, why we cannot use OCT for mounting? I think
>>sectioning would be easier.
>>Perhaps someone can help me?
I use OCT for cutting skin for IF staining. My stains come out just
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