[Histonet] IF on frozen sections

From:"Mark Adam Tarango"

>>Date: Mon, 19 Apr 2004 18:33:35 +0200
>>From: "Gudrun Lang" 
>>Subject: [Histonet] IF on frozen sections
>>To: "Histonetliste" 
>>Message-ID: <003301c4262c$0fe2b9f0$eeeea8c0@SERVER>
>>Content-Type: text/plain;	charset="iso-8859-1"

>>A question about immunfluorescence on frozen skin sections:
>>We mount the skin with buffer on the sample-table to freeze it. After
>>sectioning we let the cuts airdry and do the IF (Immunglobulins).
>>Is there any reason, why we cannot use OCT for mounting? I think
>>sectioning would be easier.
>>Perhaps someone can help me?

>>Lang Gudrun

I use OCT for cutting skin for IF staining.  My stains come out just

Mark Tarango

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