Does anyone currently use the histobath with isopentane, or a similar bath?

Currently we freeze rodent brain and spinal cords in isopentane cooled to
-65 (in a plastic beaker surrounded by dry ice). We would like to purchase a
bath to get rid of the need for dry ice, and to more easily control the
temperature.

I know that Thermo Electron (Shandon) currently makes a histobath, but I
understand that the temperature is not controllable? Is this true? Does
anyone know of any alternatives. We just need something for a benchtop,
nothing too large, and probably under 5K.

Thanks,

Rebecca Nishi
UC Irvine


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