[Histonet] About Respiratory cytologies
|From:||"María Teresa Domínguez" |
Today I received a Bronchial washing specimen (3 ml) in
the lab. We don't usually performe these kind of specimens.
The sample was collected tree days ago in the Ushuaia's Hospital
,300km away,and sent it to this Hospital (RíoGrande's Hospital), this
morning without any fixative.
Dam! I said... What should I do first?
I put the sample in the cytocentrifuge chambers with Saccommanno's
fixative, and let them there for 10 min at 1500rpm.
Now my quiestion is "What happen if I would fill the chambers with
the bronchial washing and Salt Solution equal parts? How come avoid
the cellular distortion?
The result was not so bad. My boss saw the cells, but just a few of
them. I don't know if it was the right amount ,or if it was just the
very resistents cells.
A comment it will be apreciated.
Ht. Maria Teresa Dominguez
Río Grande Reg. Hospital,
Tierra del Fuego, Argentina.
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