FW: [Histonet] Microwave Processing
|From:||"Marshall Terry Dr, Consultant Histopathologist" |
Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
Rotherham General Hospital
From: Steven E. Slap [mailto:email@example.com]
Sent: 20 April 2004 14:55
To: Marshall Terry Dr, Consultant Histopathologist
Subject: RE: [Histonet] Microwave Processing
Hi Terry & HistoNetters
Yes, in the Leong method for biopsies, the specimens are heat
stabilized in the microwave in saline, and not really chemically
fixed. They get fixed in the ethanol in a traditional processor
(Leong used ethanol, chloroform and paraffin- no xylene). I haven't
seen the resulting morphology myself.
As for breasts, the Milestone unit has a module, the DDG, which is
280mm x 250mm, for stabilization of breasts and other large organs.
One advantage with breasts is that the nodes just light up bright
Parenthetically, I don't believe that the breasts in Yorkshire could
possibly be bigger than in the American South...
>Thanks Steven. It was the Leong method to which I referred.
>I am totally befuddled over fixation in the microwave. Surely in the
>Leong method (for blocks), the saline is irrelevent other than as a
>carrier and buffer medium, and it is heat fixation?
>Firming up breasts in the microwave seems fun - but the breasts in
>Yorkshire are bigger than the microwaves:-)
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