Re: [Histonet] tissue processing


OOooh - Flashback.  I remember about 12 years ago having the exact same 
problem with the VIP and running over 100 biopsies with blue pads.  The 
blue biopsy pads retain about 1 mL of solution - 2 biopsy pads per 
cassette - 2 mL retained solution - 100 cassettes - 200 ml of retained 
solution that doesn't get rinsed out well by the next solution- so Gudrun 
- your hypothesis is correct (per my experience).  You could try a thinner 
biopsy pad - Surgipath's biopsy pads are about 1/2 the thickness of other 
vendors.  At the time we just quit using biopsy pads and went back to lens 
paper to wrap biopsies - and the problem was gone.  Also gone was a biopsy 
pad artifact from putting semi-fixed tissues (e.g. currettings) between 
blue pads. 

Jacqueline M. O'Connor HT(ASCP)
Abbott Laboratories
Global Pharmaceutical Research and Development
Discovery Chemotherapeutics

"Gudrun Lang" 
Sent by:
04/01/2004 04:10 AM

        To:     "Histonetliste" 
        Subject:        [Histonet] tissue processing

We have a current problem with our routine tissue processing in the VIP. 
We loaded 200 capsules in the container. This time we had the half of the 
capsules filled with biopsy-sponges. Usually they are only about 30% of 
all. We do over night processing.
The tissue surface was something like creamy and in the trimmed block the 
tissue looked wet. I was not able to get a good section (only holes in 
paraffin). But not all blocks were like this, most of the small biopsies 
worked well.
My suggestion is, that the great amount of sponges is responsible for the 
underprocessing. I think the tissue was'nt dehydratet enough and water was 
taken over from one step to the next.

Did anybody have the same experience? Please give me some input on this 

thanks in advance
Gudrun Lang
Akh Linz, Austria 

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