Re: [Histonet] microwave antigen retrieval
Hi HistoNetters
Successful antigen retrieval depends on accurate
control of three factors: time, temperature and
pH. Any instrument which will produce this
accuracy in a reproducible way will be effective.
A microwave has been shown to work well over a
wide range of antigens, where other methods have
required more aggressive treatment. In some
cases, pressure is needed to allow for
temperatures above 98°C. I can get most antigens
to "work" with pH 6.0 citrate buffer at 110°C in
a 3-5 minute microwave method using the
Hacker/Milestone microwave.
best regards,
Steven Slap
Microwave Consultant
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