Hi!
Are you working on human or animal tissue? In human renal biopsies most
commonly used technique is probably immunofluorescence on snap frozen
tissue. We use FITC conjugated antibodies from DakoCytomation for heavy
chains IgA, IgG and IgM in addition to C3complement, fibrinogen, kappa &
lambda light chains and albumin. These can be demonstrated also on FFPE
tissue, but the method is more involved and takes more time to develop to
obtain reproducible results. Immunofluorescence is a long standing and a
simple technique to perform...
Katri

Katri Tuomala
Department of Pathology
St.Joseph's Health Care
Hamilton, Ontario, Canada
----- Original Message ----- 
From: "ÊçÇí Éò" 
To: 
Sent: Thursday, April 08, 2004 10:36 AM
Subject: [Histonet] heavy - chain deposition desease


> Hello,everobody:
>      I am very interested in the diagonosis of heavy-chain deposition
disease.Firstly ,I am going to find out whether there are deposition of
heavy chains  along  tubular and glomerular basement membranes.But I have no
idea about which company's antibodyies are  appropriate.Would anybody be
kind enough to share me with you experieace?
> Thanks a lot.
> Beat Regards!
> Shuqiong Shen
> Research Insititute of Nephrology
> 305 East Zhongshan Road
> Nanjing 210002,P.R.China
>
>
>
>
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