Re: [Histonet] Microwave for antigen retrieval

From:"Joelle Alcock"

In a couple of methods I still am still using the microwave for antigen retrieval. For one I microwave my paraffin sections in 1L 0.01M citrate buffer 6.4pH for 25 minutes. However I have found similar results by using the abcam enzymatic antigen retrieval kit which also takes less time in all - but care must be taken as sections are more likely to crack/disintergrate.
Joelle Alcock

>>> "Featherstone, Annette"  03/31/04 12:03pm >>>
Are people still using the microwave for anitgen retrieval? I thought
steamers and pressure cookers pretty much replaced that method.
Annette Featherstone HT/MLT
Kaleida Health
Buffalo General Hospital
100 High St
Buffalo NY 14203

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