Re: [Histonet] Help with frozen section staining
Hi Pierre-André (neighbor),
I don't see the OCT being the most likely culprit, unless MAYBE if
you were perfusing with it. The OCT should only be surrounding
your tissue sections (not entering into them). I would be more
inclined to look at:
a) the fixative (try brand new batch, if the fixative is faulty, your
sections will not tolerate the non-isotonic and perhaps acid or basic
pH of your staining solutions). And then
b) the mounting medium (for clarity) and the coverslips for defects.
(presumably you've ruled out the microscope as the problem?)
Date sent: Fri, 02 Apr 2004 08:28:01 -0400
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Subject: [Histonet] Help with frozen section staining
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> Hi all,
> We just recently notice a major problem with our frozen section
> staining. The cellular morphology is poor, haziness and no cellular
> definition. We have tried different staining techniques with different
> fixative but no luck. Could it be our OCT? We desperately need some
> input on the subject. Thank you!
> Pierre-André Noel
> Histology Supervisor
> Bathurst Regional Hospital
> NB, Canada
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