Re: [Histonet] Fixation for lectin work

From:Pablo =?iso-8859-1?Q?S=E1nchez?= Quinteiro

John, I am glad to give you my input. It is constrained to my experience in
nervous tissue, testis and nasal cavity of mammals as well as in the brain
of fishes. I have got good results with Paraformaldehyde just in fishes.
Bouin worked superbly in any case. As Barry points there is huge variations
depending on the tissue, lectin and even on the species. For that reason I
counsel always to try the widest possible number of fixatives. Barry, on
what tissue did you do your study? 



At 01:16 p.m. 30/03/04 -0600, you wrote:
>Dear Pablo Sanchez
>Thank you for responding.  Could you expand a bit on your statement that
>"aldehydes work in some tissues"?
>I appreciate you time and help in this.
>John Carroll Dennis
>Anatomy, Physiology, and Pharmacology
>109 Greene Hall
>Auburn University, AL  36849

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