Re: [Histonet] Fixation for lectin work

From:Pablo =?iso-8859-1?Q?S=E1nchez?= Quinteiro

I do not know the reason, but I remember that few years ago that fact
caused me chronic headache. Just after switching to Acetic-Alcohol fixed
material I got nice labelling. Bouin fluid is also an excellent fixative
for lectin studies.

Sometimes aldehydes work in some tissues, so before working with lectins it
is advisable to compare different fixatives.


Pablo Sanchez

At 12:04 p.m. 30/03/04 -0600, you wrote:
>Dear Histonetters:
>Does anyone know why 10% buffered formaldehyde may not be suitable for 
>lectin labeling?
>John C. Dennis
>Histonet mailing list

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