Re: [Histonet] Decalcifying mouse heads

From:Pablo =?iso-8859-1?Q?S=E1nchez?= Quinteiro

Currently we are employing in adult mice whole heads "TBD-1
Rapid-decalcifier" from Shandon. At room temperature and with gentle
shaking it takes about five hours. Better to check periodically with a
blade. We have not yet used this samples for immunohistochemistry, just for
lectin histochemical labelling and it works.



At 05:33 p.m. 30/03/04 -0600, you wrote:
>We are interested in decalcifying whole heads from adult mice in order to
examine histological sections throughout the oral cavity and esophagus. Are
you aware what would be appropriate for such a large piece of tissue, or
how long it would take to decalcify? In the future we would like to have
the option of performing immunohistochemistry on our decalcified samples.
Do you have any experience with IHC on such large decalcified samples? 
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