Re: [Histonet] Decalcifying mouse heads
We used formic acid to decalcify our mouse heads. They didn't take too
long (1 week) although I cant remember how long precisely. And yes they
were used for immuno studies. We had a few studies, one for temporal bones
and another for the whole head. They worked fine.
We are interested in decalcifying whole heads from adult mice in order to
examine histological sections throughout the oral cavity and esophagus. Are
you aware what would be appropriate for such a large piece of tissue, or
how long it would take to decalcify? In the future we would like to have
the option of performing immunohistochemistry on our decalcified samples.
Do you have any experience with IHC on such large decalcified samples?
Histology Service Unit
University of Otago
(03) 479 7152
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