RE: [Histonet] detach cells from a slide....again

From:"Favara, Cynthia (NIH/NIAID)"

Proteinase K at a high conc. should do it. 


Cynthia Favara
903 South 4th Street
Hamilton, MT 59840

-----Original Message-----
From: Dolors Fuster []
Sent: Wednesday, April 14, 2004 10:50 AM
Subject: [Histonet] detach cells from a slide....again

In my previous mail I forgot to tell you the mechanical method (scrape them
with a sharp razor blade) is made....but too slow for at least 600 
slides...difficult to recover all stuff...some of recovered adheres to the 
eppendorf.... :o(

Could the mehod not include trypsin in its procedure :o) ?? 

Thanks again

Any idea to detach cells from an hematologic slide?

They are dryed...stained with May GrŘnwald Giemsa...dehidrated, cleared and 
mounted with DPX.

The idea is to use them for a DNA extraction.

Thanks in advance

Histonet mailing list

----- Fi del missatge reenviat -----

Dolors Fuster
TŔcnic Especialista en Anatomia Patol˛gica i Citologia
Departament d'Anatomia i Embriologia Humana 
Facultat de Medicina (HCP) 
Universitat de Barcelona 
ICQ 14146798 

This mail sent through IMP:

Histonet mailing list

Histonet mailing list

<< Previous Message | >>