RE: [Histonet] detach cells from a slide....again

From:"Favara, Cynthia (NIH/NIAID)"

Proteinase K at a high conc. should do it. 

c

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317


-----Original Message-----
From: Dolors Fuster [mailto:d.fuster@ub.edu]
Sent: Wednesday, April 14, 2004 10:50 AM
To: HISTONET
Subject: [Histonet] detach cells from a slide....again


In my previous mail I forgot to tell you the mechanical method (scrape them
off 
with a sharp razor blade) is made....but too slow for at least 600 
slides...difficult to recover all stuff...some of recovered adheres to the 
eppendorf.... :o(

Could the mehod not include trypsin in its procedure :o) ?? 

Thanks again



Any idea to detach cells from an hematologic slide?

They are dryed...stained with May GrŘnwald Giemsa...dehidrated, cleared and 
mounted with DPX.

The idea is to use them for a DNA extraction.

Thanks in advance



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----- Fi del missatge reenviat -----


Dolors Fuster
TŔcnic Especialista en Anatomia Patol˛gica i Citologia
Departament d'Anatomia i Embriologia Humana 
Facultat de Medicina (HCP) 
Universitat de Barcelona 
ICQ 14146798 



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