I really doubt you would lose vegf antigenicity, usually the problem with vegf is that it is so over expressed that it stains too heavily.  Perhaps old samples may look better than fresh one's in this case.
Patsy

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Mass
Histology Service
Sent: Saturday, April 10, 2004 7:10 PM
To: Sebree Linda A.; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] VEGF


Linda,

The slides were sent to us, so I have no way of knowing their age.  The surgical numbers were from several years back and the glass slides were from various manufacturers.  They all stained the same intensity.

By the way, I don't charge for my time in answering questions from anyone who asks for help.

Jim 

____________________________
  James E. Staruk, HT(ASCP)
   Mass Histology Service
   www.masshistology.com 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Sebree
Linda A.
Sent: Saturday, April 10, 2004 5:09 PM
To: Mass Histology Service; Patsy Ruegg; Amos Brooks;
histonet@lists.utsouthwestern.edu
Cc: Yracheta Joseph
Subject: RE: [Histonet] VEGF


Jim,
 
Did you find there was a problem with oxidation?  A researcher has brought us a number of renal biopsy slides for VEGF staining, some which have been cut for a couple years and I presume stored at R. T., and I'm afraid we may have an issue with antigenicity of the epitope.  What do you think?
 
Linda Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory



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