Actually no,
         The study we were doing was using blocks that we had cut freshly. 
The blocks were old (mostly) but they were generally cut and stained within 
a week. I have seen degradation of epitopes in old slides but not in old 
blocks recently cut. Truly the culprit was a very poor primary antibody. 
I'll certainly be looking into the monoclonal Tomorrow morning though.
Hoping for the best,
Amos

At 04:09 PM 4/10/2004, you wrote:
>Jim,
>
>Did you find there was a problem with oxidation?  A researcher has brought 
>us a number of renal biopsy slides for VEGF staining, some which have been 
>cut for a couple years and I presume stored at R. T., and I'm afraid we 
>may have an issue with antigenicity of the epitope.  What do you think?
>
>Linda Sebree
>University of Wisconsin Hospital & Clinics
>IHC/ISH Laboratory



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