RE: [Histonet] VEGF

From:"Sebree Linda A."

Did you find there was a problem with oxidation?  A researcher has brought us a number of renal biopsy slides for VEGF staining, some which have been cut for a couple years and I presume stored at R. T., and I'm afraid we may have an issue with antigenicity of the epitope.  What do you think?
Linda Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory

	-----Original Message----- 
	From: Mass Histology Service [] 
	Sent: Fri 4/9/2004 3:57 PM 
	To: Patsy Ruegg; Amos Brooks; 
	Subject: RE: [Histonet] VEGF

	We just completed a large study on human prostate biopsies using the Santa
	Cruz monoclonal VEGF (C1) following pronase pre-treatment and they all came
	out  wonderful:  Very clean and the negative controls (no primary) were
	completely negative.
	James Staruk, HT(ASCP)
	Mass Histology Service
	-----Original Message-----
	[]On Behalf Of Patsy
	Sent: Friday, April 09, 2004 3:38 PM
	To: Amos Brooks;
	Subject: RE: [Histonet] VEGF
	I totally agree with you about Santa Cruz's poly VEGF but the mono was much
	better.  I have a customer who still wants to use the poly vegf on sheep
	placenta and I think it is awful but you have to give the customer what they
	want.  I did a comparison with the poly and mono for them but they still
	opted for the poly because it matched their molecular studies.  Oh well.  To
	me it is all over the place but they seem to think that is expected for
	their samples, they think is is real and call it "an expression antibody" or
	something like that.
	Histonet mailing list

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