RE: [Histonet] Safranine o staining and Problem in tissues
What are you looking for in the joint staining you want? If you want
something with safranine O, Movat's Pentachrome comes to mind. Not
having worked with bone histology for over 20 years, I'm not the best
resource for this. I do remember Movat's being a big favorite during
the time I worked specifically in bone histology. It was done on
decalcified bone as well a MMA embedded bones.
Decal-Stat from the Decal Corp. PO Box 236, Congers, NY 10920;
Phone: 800/428-5856; fax: 845/268-383
This is a rapid decalcifier. We use it on all our tissues regardless of
anmal. It works great.
As for your liver, kidney and brain sections, --- I'm going out on a
limb here --- but it sounds like it could be heat artifact. Are you
putting your sections on a heating tray before staining them? If so,
check the temperature... it may be way too hot. My water bath is set at
37 to 38 C and I keep my heating tray set to 40 C. That's pretty warm,
but it works well for tissues like gut, heart, skin, ovary, uterus
kidney, liver. I don't leave any tissue on this tray for more than 10
or 15 minutes and haven't had any problems like this. Generally, I
don't put brain, or fetal tissues, anything frail on the tray except if
they seem to need a little relaxing of the tissue. In that case, I
watch the sections carefully and remove them as soon as they smooth out.
For what it's worth...
Utah Veterinary Diagnostics Laboratory
Utah State University
[mailto:email@example.com] On Behalf Of
Sent: Tuesday, April 13, 2004 9:15 PM
Subject: [Histonet] Safranine o staining and Problem in tissues
Can anyone give me the detailed procedure for safranine o staining for
joints (ankle. tarsal and metatarsal joints in rats). I would like to
a good decalcifying solution for the same and what is the duration of
bone to be kept in the decalcifier.
Secondly, of late I am facing peculiar problem now with my liver,
and brain sections of late. These sections are cracking after the
(Mayer's H& E). It is something like parched earth appearance. This I am
noticing when the tissue s are coming to last xylenes. I donot see the
cracking before this step. This doesn't happen in all the slides. Could
you please suggest some solution for this. We are using chloroform for
clearing and histoplast from Shandon for infiltration. I am unable to
locate where exactly is the problem. I have tried changing the makes of
chloroform and xylenes used with out much help.
Dr. Balaji (M. V. Sc. Path)
Dept. Pre clinical safety evaluation,
Dr. Reddys Laboratories ltd. Bollaram Road,
Miyapur, Hyderabad, 500 049
Andhra Pradseh, INDIA
Tel: 23045439 - Ext.432.
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