RE: Fwd: Re: [Histonet] Decalcifying mouse heads

From:Caroline Stott

We use 10% formic acid.  We use the chemical test to check if 
decalcification is complete.  That is 5ml of ammonium hydroxide added to 
5ml of the bone solution and add 5ml saturated ammonium oxalate.  If the 
solution is milky white, there is still calcium present.  So keep changing 
the solution every second day until it is finished.

Caroline Stott

Histology Service Unit
Medical School
University of Otago
(03) 479 7152 

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