[Histonet] porcine insulin
Hello everybody,
I'm hoping someone can give me any suggestions on how to detect insulin on a
(paraffin embedded formalin-fixed or frozen) porcine section using the
following antibodies:
- primary antibody: Guinea Pig anti-insulin (Dako)
- secondary antibody: Swine anti-Rabbit (Dako)
According to the DAKO catalogous the anti-Rabbit should work in this combo.
I have tried it over and over again, using different protocols, but somehow
I just can't get the job done...
Thanks in advance!
Samantha
s.stryker@lumc.nl
Leiden University Medical Centre
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