[Histonet] porcine insulin

From:"Stryker, S. (HLK)"

Hello everybody,

I'm hoping someone can give me any suggestions on how to detect insulin on a
(paraffin embedded formalin-fixed or frozen) porcine section using the
following antibodies:

- primary antibody: Guinea Pig anti-insulin (Dako)
- secondary antibody: Swine anti-Rabbit (Dako)

According to the DAKO catalogous the anti-Rabbit should work in this combo.
I have tried it over and over again, using different protocols, but somehow
I just can't get the job done... 

Thanks in advance!

Leiden University Medical Centre

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