[Histonet] bcl-2 on Nexes

From:"Derek & Lynda Leopold"

Hi All,
We're having some trouble getting our tonsil control to come up for bcl-2.  After searching the archives and asking around, we are going to try citrate buffer (Declere) and a hot steamer for 1 hour.  We've got the Nexes IHC.  Anyone else have any tips for us?
Can anyone comment on the need/non-need for DAB?
Thanks
Lynda Leopold
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