[Histonet] Toluidine Blue
We have used the same stain protocols for years for mast cells (metachromatic staining) and now we have some problems.
We have received a new batch of salt from Polysciences
(Have used the salt from Polyscinces Toluidine Blue O C.I 52040 purified for years)
Stain protocols we have done is 1% Toluidine blue solved in 20% ethanol 20 minutes.
Rinse in 95% ethanol
Dehydrate quickly through 95% Abs. alcohols
Clear in Xylene.
The metachromatic is not purple the are pale blue or even blue-green.
Hope for some input.
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