We have used the same stain protocols for years for mast cells (metachromatic staining) and now we have some problems.

We have received a new batch of salt from Polysciences

(Have used the salt from Polyscinces Toluidine Blue O C.I 52040 purified for years)

 

Stain protocols we have done is 1% Toluidine blue solved in 20% ethanol 20 minutes.

Rinse in 95% ethanol 

Dehydrate quickly through 95% Abs. alcohols 

Clear in Xylene.

 

 

The metachromatic is not purple the are pale blue or even blue-green.

 

 

Hope for some input.

 

 

BioVet AB

 

 

Britt-Marie Smedberg

 

biovetlab@biovet.se

 
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