[Histonet] Submit
Topic: microtome
Dear histonet friends,
We are seting up a histological lab. Currently we are looking for a woring
microtone for paraffine block tissue sectioning. If someone can help, please
contact wirh me. Thanks.
Ze Lu, Dr.
Optimum Therapeutics, LLC
Email: optimum-t@columbus.rr.com
----- Original Message -----
From:
To:
Sent: Tuesday, April 13, 2004 12:01 PM
Subject: Histonet Digest, Vol 5, Issue 20
> Send Histonet mailing list submissions to
> histonet@lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
> histonet-request@lists.utsouthwestern.edu
>
> You can reach the person managing the list at
> histonet-owner@lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
> 1. RE: histogel (S Ladd)
> 2. Re: Vibratome Considerations (Geoff McAuliffe)
> 3. Histology Manager Opening at Yale University (Ramona Tolliver)
> 4. RE: histogel (Andrea Grantham)
> 5. Zinc formalin (Lorraine Cornett)
> 6. RE: histogel (Connie McManus)
> 7. RE: histogel (S Ladd)
> 8. RE: histogel (S Ladd)
> 9. RE: histogel (Bartlett, Jeanine)
> 10. RE: histogel (Bartlett, Jeanine)
> 11. RE: histogel (Patsy Ruegg)
> 12. Rat Turbinates (Antonia Abeyta)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Tue, 13 Apr 2004 10:49:12 -0400
> From: "S Ladd"
> Subject: RE: [Histonet] histogel
> To: "m. van mkempen"
> Cc: histonet@lists.utsouthwestern.edu
> Message-ID:
> Content-Type: text/plain; charset="US-ASCII"
>
> Yes Yes Yes!!!!!!! If you don't fix the histogel in formalin before
> processing it WILL DISSOLVE! I learned this the hard way! The instructions
> say something vague like "process as usual". Of course, I informed Richard
> Allen that many of us in research do not have formalin on our tissue
> processors. Richard Allen said they were going to change the instructions.
> Once you have fixed the histogel you can process it like you would any
other
> piece of tissue.
> Sharron
> University of South Florida
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of m. van
> mkempen
> Sent: Tuesday, April 13, 2004 10:18 AM
> To: Histonetlist
> Subject: [Histonet] histogel
>
>
> Dear all,
>
> I have a question concerning histogel!!
> I'm working with fetal lung and I thought histogel would be a nice way
> to handle then while embedding.
> My question is do I have to fix the histogel before processing for
> embedding in paraffine?
> How is the normal procedure for tissues's in histogel??
>
> Thank you for all your kind answers.
>
> Regards,
> Marta.
>
> --
> ----------------------------
> *- mkempen -*
> MAILTO:m.vankempen@erasmusmc.nl
> -----------------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 2
> Date: Tue, 13 Apr 2004 11:02:38 -0700
> From: Geoff McAuliffe
> Subject: Re: [Histonet] Vibratome Considerations
> To: Danielle Zalinski
> Cc: HistoNet Listserve
> Message-ID: <407C2B3E.40602@umdnj.edu>
> Content-Type: text/plain; format=flowed; charset=us-ascii
>
> I have done immunos on vibratome sections of rat brain. If you use a 12
> well tissue culture plate (with its lid) on a shaker table overnight at
> room temp. you should get good results. You will need to add some Triton
> or Tween to the primary antibody (and probably subsequent steps) or do
> something to 'permeabilize' the sections (freeze-thaw or alcohol) so you
> get good penetration of the antibodies. If you have access to a sliding
> microtome with a freezing stage you could obtain sections that way.
>
> Geoff
>
> Danielle Zalinski wrote:
>
> > Hello All,
> >Our lab would like to do immunohistochem work in rat brain on vibratome
> >sections instead of paraffin. We are able to get sections about
> >50-60um. Will incubation with primary antibody overnight be sufficient?
> > Is shaking the sections an option? I was hoping some other labs that
> >are working in vibratome sections may have some beginners advice.
> >Thanks,
> >Danielle Zalinski
> >
> >Neurosurgery Research
> >Henry Ford Health System
> >Detroit, MI
> >
> >
> >CONFIDENTIALITY NOTICE: This email contains information from the sender
that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise
protected from disclosure. This email is intended for use only by the person
or entity to whom it is addressed. If you are not the intended recipient,
any use, disclosure, copying, distribution, printing, or any action taken in
reliance on the contents of this email, is strictly prohibited. If you
received this email in error, please contact the sending party by replying
in an email to the sender, delete the email from your computer system and
shred any paper copies of the email you printed.
> >
> >Note to Patients: There are a number of risks you should consider before
using e-mail to communicate with us. These risks are described in our
Privacy Policy at http://henryford.com. Review that policy carefully before
continuing to communicate with us by e-mail. For greater Internet security,
our policy describes the Henry Ford MyHealth electronic communication
process - you may register at http://henryford.com. If you do not believe
that our policy gives you the privacy and security protection you need, do
not send e-mail or Internet communications to us.
> >_______________________________________________
> >Histonet mailing list
> >Histonet@lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
>
> --
> --
> **********************************************
> Geoff McAuliffe, Ph.D.
> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
> 675 Hoes Lane, Piscataway, NJ 08854
> voice: (732)-235-4583; fax: -4029
> mcauliff@umdnj.edu
> **********************************************
>
>
>
>
>
>
> ------------------------------
>
> Message: 3
> Date: Tue, 13 Apr 2004 11:14:18 -0400
> From: Ramona Tolliver
> Subject: [Histonet] Histology Manager Opening at Yale University
> To: histonet@lists.utsouthwestern.edu
> Message-ID: <6.0.1.1.2.20040413111131.01e75d48@email.med.yale.edu>
> Content-Type: text/plain; charset="us-ascii"; format=flowed
>
> Good morning,
>
> Yale University has a Histology Manager (1st shift) position available
from
> 7:00 a.m. - 3:00 p.m. If you know of anyone who may be interested in an
> outstanding growth opportunity in a department committed to excellence in
> patient care, teaching, and discovery, please forward this information on
> to them.
>
> Salary is commensurate with experience. Relocation assistance is
> available. The position is posted online at :
> http://websrv.its.yale.edu/hr/cgi-bin/printjob.plx?file=mp-2&job=11326 .
>
> Inquiries should be sent to ramona.tolliver@yale.edu. Thank you for your
time!
>
>
>
> Ramona E. Tolliver
>
> *If you have difficulty with the link provided above, please go to
> www.yale.edu/jobs and search Managerial and Professional last two weeks
and
> Pathology. The position is listed as Manager III.
>
>
>
>
> Ramona E. Tolliver
> Human Resource Manager
> Yale University School of Medicine
> Department of Pathology
> 310 Cedar Street
> New Haven, CT 06520-8023
> Telephone: (203) 785-6689
> Fax: (203) 785-7303
>
> ------------------------------
>
> Message: 4
> Date: Tue, 13 Apr 2004 08:28:11 -0700
> From: Andrea Grantham
> Subject: RE: [Histonet] histogel
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
> <4.3.2.7.2.20040413082229.00cb9f10@algranth.inbox.email.arizona.edu>
> Content-Type: text/plain; charset="us-ascii"; format=flowed
>
> When I use histogel I do "process as usual" and this does not include
> formalin. I put the cassette containing the tissue embedded in histogel
> into 70% ETOH. The processor starts with 70% ETOH X 2 and the
concentration
> of ETOH goes up from there. Haven't had any problems.
> Maybe I've just been lucky?
> Andi Grantham
>
>
>
> At 10:49 AM 4/13/2004 -0400, you wrote:
> >Yes Yes Yes!!!!!!! If you don't fix the histogel in formalin before
> >processing it WILL DISSOLVE! I learned this the hard way! The
instructions
> >say something vague like "process as usual". Of course, I informed
Richard
> >Allen that many of us in research do not have formalin on our tissue
> >processors. Richard Allen said they were going to change the
instructions.
> >Once you have fixed the histogel you can process it like you would any
other
> >piece of tissue.
> >Sharron
> >University of South Florida
> >
> >-----Original Message-----
> >From: histonet-bounces@lists.utsouthwestern.edu
> >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of m. van
> >mkempen
> >Sent: Tuesday, April 13, 2004 10:18 AM
> >To: Histonetlist
> >Subject: [Histonet] histogel
> >
> >
> >Dear all,
> >
> >I have a question concerning histogel!!
> >I'm working with fetal lung and I thought histogel would be a nice way
> >to handle then while embedding.
> >My question is do I have to fix the histogel before processing for
> >embedding in paraffine?
> >How is the normal procedure for tissues's in histogel??
> >
> >Thank you for all your kind answers.
> >
> >Regards,
> >Marta.
> >
> >--
> >----------------------------
> >*- mkempen -*
> >MAILTO:m.vankempen@erasmusmc.nl
> >-----------------------------------------
> >
> >_______________________________________________
> >Histonet mailing list
> >Histonet@lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >_______________________________________________
> >Histonet mailing list
> >Histonet@lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> .....................................................................
> : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
> : Sr. Research Specialist University of Arizona :
> : (office: AHSC 4212) P.O. Box 245044 :
> : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
> : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) :
> :...................................................................:
> http://www.cba.arizona.edu/histology-lab.html
>
>
>
>
> ------------------------------
>
> Message: 5
> Date: Tue, 13 Apr 2004 11:32:55 -0400
> From: "Lorraine Cornett"
> Subject: [Histonet] Zinc formalin
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
> Content-Type: text/plain; format=flowed
>
> We are currentyl processing our biopsies in 10% neutral buffered formalin
> (short process cycle). Our pathologists miss the nuclear detail they
became
> accustomed to when we were processing in Zinc Formalin. We seem to
remember
> a procedure where you can "mordant" or post fix the actual slides before
> staining. Does anyone have this procedure that they can share with me.
>
> Thanks,
>
> Lorraine Cornett (HT ASCP)
> Blue Ridge Pathology
> Kingsport, TN 37660
> 423 224-5793
>
> _________________________________________________________________
> Is your PC infected? Get a FREE online computer virus scan from McAfeeŽ
> Security. http://clinic.mcafee.com/clinic/ibuy/campaign.asp?cid=3963
>
>
>
>
> ------------------------------
>
> Message: 6
> Date: Tue, 13 Apr 2004 09:24:43 -0600
> From: "Connie McManus"
> Subject: RE: [Histonet] histogel
> To: "'S Ladd'" , "'m. van mkempen'"
>
> Cc: histonet@lists.utsouthwestern.edu
> Message-ID: <000101c4216b$72f8c5e0$4a737b81@Cygnus>
> Content-Type: text/plain; charset="us-ascii"
>
> Forgive my ignorance, but what is histogel and what is it used for?
> thanx
>
> Connie McManus
> Utah Veterinary Diagnostics Laboratory
> Utah State University
> Logan, UT
> Phone: 435/797-1891
> fax: 435/797-2805
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of S Ladd
> Sent: Tuesday, April 13, 2004 7:49 AM
> To: m. van mkempen
> Cc: histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] histogel
>
> Yes Yes Yes!!!!!!! If you don't fix the histogel in formalin before
> processing it WILL DISSOLVE! I learned this the hard way! The
> instructions
> say something vague like "process as usual". Of course, I informed
> Richard
> Allen that many of us in research do not have formalin on our tissue
> processors. Richard Allen said they were going to change the
> instructions.
> Once you have fixed the histogel you can process it like you would any
> other
> piece of tissue.
> Sharron
> University of South Florida
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of m. van
> mkempen
> Sent: Tuesday, April 13, 2004 10:18 AM
> To: Histonetlist
> Subject: [Histonet] histogel
>
>
> Dear all,
>
> I have a question concerning histogel!!
> I'm working with fetal lung and I thought histogel would be a nice way
> to handle then while embedding.
> My question is do I have to fix the histogel before processing for
> embedding in paraffine?
> How is the normal procedure for tissues's in histogel??
>
> Thank you for all your kind answers.
>
> Regards,
> Marta.
>
> --
> ----------------------------
> *- mkempen -*
> MAILTO:m.vankempen@erasmusmc.nl
> -----------------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 7
> Date: Tue, 13 Apr 2004 11:42:46 -0400
> From: "S Ladd"
> Subject: RE: [Histonet] histogel
> To: "Andrea Grantham"
> Cc: Histonet@lists.utsouthwestern.edu
> Message-ID:
> Content-Type: text/plain; charset="US-ASCII"
>
> Are the items you are embedding in the histogel fixed in formalin?
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Andrea
> Grantham
> Sent: Tuesday, April 13, 2004 11:28 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] histogel
>
>
> When I use histogel I do "process as usual" and this does not include
> formalin. I put the cassette containing the tissue embedded in histogel
> into 70% ETOH. The processor starts with 70% ETOH X 2 and the
concentration
> of ETOH goes up from there. Haven't had any problems.
> Maybe I've just been lucky?
> Andi Grantham
>
>
>
> At 10:49 AM 4/13/2004 -0400, you wrote:
> >Yes Yes Yes!!!!!!! If you don't fix the histogel in formalin before
> >processing it WILL DISSOLVE! I learned this the hard way! The
instructions
> >say something vague like "process as usual". Of course, I informed
Richard
> >Allen that many of us in research do not have formalin on our tissue
> >processors. Richard Allen said they were going to change the
instructions.
> >Once you have fixed the histogel you can process it like you would any
> other
> >piece of tissue.
> >Sharron
> >University of South Florida
> >
> >-----Original Message-----
> >From: histonet-bounces@lists.utsouthwestern.edu
> >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of m. van
> >mkempen
> >Sent: Tuesday, April 13, 2004 10:18 AM
> >To: Histonetlist
> >Subject: [Histonet] histogel
> >
> >
> >Dear all,
> >
> >I have a question concerning histogel!!
> >I'm working with fetal lung and I thought histogel would be a nice way
> >to handle then while embedding.
> >My question is do I have to fix the histogel before processing for
> >embedding in paraffine?
> >How is the normal procedure for tissues's in histogel??
> >
> >Thank you for all your kind answers.
> >
> >Regards,
> >Marta.
> >
> >--
> >----------------------------
> >*- mkempen -*
> >MAILTO:m.vankempen@erasmusmc.nl
> >-----------------------------------------
> >
> >_______________________________________________
> >Histonet mailing list
> >Histonet@lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >_______________________________________________
> >Histonet mailing list
> >Histonet@lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> .....................................................................
> : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
> : Sr. Research Specialist University of Arizona :
> : (office: AHSC 4212) P.O. Box 245044 :
> : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
> : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) :
> :...................................................................:
> http://www.cba.arizona.edu/histology-lab.html
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 8
> Date: Tue, 13 Apr 2004 11:42:45 -0400
> From: "S Ladd"
> Subject: RE: [Histonet] histogel
> To: "Kyle-Byrne, Carrie - Labvision"
> Cc: histonet@lists.utsouthwestern.edu
> Message-ID:
> Content-Type: text/plain; charset="iso-8859-1"
>
> Since your cells were fixed in formalin, perhaps there was enough residual
> formalin to sufficiently fix the histogel? You use only a few drops of
> histogel. My cells were not in formalin and I use about 0.5 mL of
histogel.
> Just for fun, you could try processing a little button of histogel
(without
> formalin fixed cells) and see if it dissolves?
> I use ethanol and xylene on my processor and I also start with 70%
ethanol.
> Sharron
>
> -----Original Message-----
> From: Kyle-Byrne, Carrie - Labvision [mailto:CKByrne@labvision.com]
> Sent: Tuesday, April 13, 2004 11:23 AM
> To: 'S Ladd'
> Subject: RE: [Histonet] histogel
>
>
> Sharron,
> Interesting. I've never heard of this before. I've used Histogel for
years
> and never had to 'fix' it in formalin. I used it to make cell pellets for
> use as IHC control material. I would fix the cells in formalin first then
> transfer to 70% EtOH and hold them until I had several to pellet. I'd
> decant the 70%, put on a few drops of Histogel, mix to distribute the
cells,
> then cool the tube to harden the gel. Once solidified, I'd remove the
> pellet from the tube and breadloaf it like a piece of tissue, place in a
> cassette and onto the processor (we started in 70% EtOH) for overnight
> processing and embed as usual in the morning. It always worked just fine.
> What to you use for clearing?
>
> Carrie Kyle-Byrne
> Sr. Reseach Assoc., Pathology Lab
> Lab Vision, Corp.
> 47791 Westinghouse Ave.
> Fremont, CA 94539
> ckbyrne@labvision.com
> www.labvision.com
>
>
>
> -----Original Message-----
> From: S Ladd [mailto:sladd@hsc.usf.edu]
> Sent: Tuesday, April 13, 2004 7:49 AM
> To: m. van mkempen
> Cc: histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] histogel
>
>
> Yes Yes Yes!!!!!!! If you don't fix the histogel in formalin before
> processing it WILL DISSOLVE! I learned this the hard way! The instructions
> say something vague like "process as usual". Of course, I informed Richard
> Allen that many of us in research do not have formalin on our tissue
> processors. Richard Allen said they were going to change the instructions.
> Once you have fixed the histogel you can process it like you would any
other
> piece of tissue.
> Sharron
> University of South Florida
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of m. van
> mkempen
> Sent: Tuesday, April 13, 2004 10:18 AM
> To: Histonetlist
> Subject: [Histonet] histogel
>
>
> Dear all,
>
> I have a question concerning histogel!!
> I'm working with fetal lung and I thought histogel would be a nice way
> to handle then while embedding.
> My question is do I have to fix the histogel before processing for
> embedding in paraffine?
> How is the normal procedure for tissues's in histogel??
>
> Thank you for all your kind answers.
>
> Regards,
> Marta.
>
> --
> ----------------------------
> *- mkempen -*
> MAILTO:m.vankempen@erasmusmc.nl
> -----------------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 9
> Date: Tue, 13 Apr 2004 11:43:09 -0400
> From: "Bartlett, Jeanine"
> Subject: RE: [Histonet] histogel
> To: "Andrea Grantham" ,
>
> Message-ID:
>
> Content-Type: text/plain; charset="us-ascii"
>
> We do the same and have had no problems.
>
> Jeanine Bartlett, HT(ASCP)
> Centers for Disease Control
> Infectious Disease Pathology Activity
> 1600 Clifton Road, MS/G-32
> Atlanta, GA 30333
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea
> Grantham
> Sent: Tuesday, April 13, 2004 11:28 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] histogel
>
>
> When I use histogel I do "process as usual" and this does not include
> formalin. I put the cassette containing the tissue embedded in histogel
> into 70% ETOH. The processor starts with 70% ETOH X 2 and the
> concentration
> of ETOH goes up from there. Haven't had any problems.
> Maybe I've just been lucky?
> Andi Grantham
>
>
>
> At 10:49 AM 4/13/2004 -0400, you wrote:
> >Yes Yes Yes!!!!!!! If you don't fix the histogel in formalin before
> >processing it WILL DISSOLVE! I learned this the hard way! The
> >instructions say something vague like "process as usual". Of course, I
> >informed Richard Allen that many of us in research do not have formalin
>
> >on our tissue processors. Richard Allen said they were going to change
> >the instructions. Once you have fixed the histogel you can process it
> >like you would any other piece of tissue. Sharron
> >University of South Florida
> >
> >-----Original Message-----
> >From: histonet-bounces@lists.utsouthwestern.edu
> >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of m. van
> >mkempen
> >Sent: Tuesday, April 13, 2004 10:18 AM
> >To: Histonetlist
> >Subject: [Histonet] histogel
> >
> >
> >Dear all,
> >
> >I have a question concerning histogel!!
> >I'm working with fetal lung and I thought histogel would be a nice way
> >to handle then while embedding. My question is do I have to fix the
> >histogel before processing for embedding in paraffine?
> >How is the normal procedure for tissues's in histogel??
> >
> >Thank you for all your kind answers.
> >
> >Regards,
> >Marta.
> >
> >--
> >----------------------------
> >*- mkempen -*
> >MAILTO:m.vankempen@erasmusmc.nl
> >-----------------------------------------
> >
> >_______________________________________________
> >Histonet mailing list
> >Histonet@lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >_______________________________________________
> >Histonet mailing list
> >Histonet@lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> .....................................................................
> : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
> : Sr. Research Specialist University of Arizona :
> : (office: AHSC 4212) P.O. Box 245044 :
> : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
> : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) :
> :...................................................................:
> http://www.cba.arizona.edu/histology-lab.html
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 10
> Date: Tue, 13 Apr 2004 11:47:04 -0400
> From: "Bartlett, Jeanine"
> Subject: RE: [Histonet] histogel
> To: "S Ladd" , "Andrea Grantham"
>
> Cc: Histonet@lists.utsouthwestern.edu
> Message-ID:
>
> Content-Type: text/plain; charset="us-ascii"
>
> Yes.
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of S Ladd
> Sent: Tuesday, April 13, 2004 11:43 AM
> To: Andrea Grantham
> Cc: Histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] histogel
>
>
> Are the items you are embedding in the histogel fixed in formalin?
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Andrea
> Grantham
> Sent: Tuesday, April 13, 2004 11:28 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] histogel
>
>
> When I use histogel I do "process as usual" and this does not include
> formalin. I put the cassette containing the tissue embedded in histogel
> into 70% ETOH. The processor starts with 70% ETOH X 2 and the
> concentration of ETOH goes up from there. Haven't had any problems.
> Maybe I've just been lucky? Andi Grantham
>
>
>
> At 10:49 AM 4/13/2004 -0400, you wrote:
> >Yes Yes Yes!!!!!!! If you don't fix the histogel in formalin before
> >processing it WILL DISSOLVE! I learned this the hard way! The
> >instructions say something vague like "process as usual". Of course, I
> >informed Richard Allen that many of us in research do not have formalin
>
> >on our tissue processors. Richard Allen said they were going to change
> >the instructions. Once you have fixed the histogel you can process it
> >like you would any
> other
> >piece of tissue.
> >Sharron
> >University of South Florida
> >
> >-----Original Message-----
> >From: histonet-bounces@lists.utsouthwestern.edu
> >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of m. van
> >mkempen
> >Sent: Tuesday, April 13, 2004 10:18 AM
> >To: Histonetlist
> >Subject: [Histonet] histogel
> >
> >
> >Dear all,
> >
> >I have a question concerning histogel!!
> >I'm working with fetal lung and I thought histogel would be a nice way
> >to handle then while embedding. My question is do I have to fix the
> >histogel before processing for embedding in paraffine?
> >How is the normal procedure for tissues's in histogel??
> >
> >Thank you for all your kind answers.
> >
> >Regards,
> >Marta.
> >
> >--
> >----------------------------
> >*- mkempen -*
> >MAILTO:m.vankempen@erasmusmc.nl
> >-----------------------------------------
> >
> >_______________________________________________
> >Histonet mailing list
> >Histonet@lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >_______________________________________________
> >Histonet mailing list
> >Histonet@lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> .....................................................................
> : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
> : Sr. Research Specialist University of Arizona :
> : (office: AHSC 4212) P.O. Box 245044 :
> : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
> : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) :
> :...................................................................:
> http://www.cba.arizona.edu/histology-lab.html
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 11
> Date: Tue, 13 Apr 2004 10:00:05 -0600
> From: "Patsy Ruegg"
> Subject: RE: [Histonet] histogel
> To: "S Ladd" , "m. van mkempen"
>
> Cc: histonet@lists.utsouthwestern.edu
> Message-ID:
> Content-Type: text/plain; charset="us-ascii"
>
> I use histogel all the time without fixing in formalin. Like Andi I start
> with tissue already fixed and then placed in 70% alcohol. I embed this
> tissue in histogel and put it back in the 70% and then process starting
with
> 70% alcohol, no formalin. I have not had the histogel ever dissolve.
> Patsy
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of S Ladd
> Sent: Tuesday, April 13, 2004 8:49 AM
> To: m. van mkempen
> Cc: histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] histogel
>
>
> Yes Yes Yes!!!!!!! If you don't fix the histogel in formalin before
> processing it WILL DISSOLVE! I learned this the hard way! The instructions
> say something vague like "process as usual". Of course, I informed Richard
> Allen that many of us in research do not have formalin on our tissue
> processors. Richard Allen said they were going to change the instructions.
> Once you have fixed the histogel you can process it like you would any
other
> piece of tissue.
> Sharron
> University of South Florida
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of m. van
> mkempen
> Sent: Tuesday, April 13, 2004 10:18 AM
> To: Histonetlist
> Subject: [Histonet] histogel
>
>
> Dear all,
>
> I have a question concerning histogel!!
> I'm working with fetal lung and I thought histogel would be a nice way
> to handle then while embedding.
> My question is do I have to fix the histogel before processing for
> embedding in paraffine?
> How is the normal procedure for tissues's in histogel??
>
> Thank you for all your kind answers.
>
> Regards,
> Marta.
>
> --
> ----------------------------
> *- mkempen -*
> MAILTO:m.vankempen@erasmusmc.nl
> -----------------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 12
> Date: Tue, 13 Apr 2004 10:57:18 -0600
> From: "Antonia Abeyta"
> Subject: [Histonet] Rat Turbinates
> To:
> Message-ID:
> Content-Type: text/plain; charset=US-ASCII
>
> Hi all,
>
> I am planning on staining some rat turbinates with Alcian Blue, and
> counter-staining with Hematoxylin & Eosin. Some of the literature
> suggests also adding a Periodic Acid Schiff (PAS) step into the
> protocol. What exactly does this step change and what is the PAS step
> useful for?
>
> Also, if anyone has some pointers for keeping the rat turbs from
> floating off the slides...we are currently using charged slides but they
> don't seem to be working.
>
> Thanks.
>
> Antonia L. Abeyta
> Health Sciences Tech. III
> Community Environmental Health Program
> University of New Mexico
> Surge Bldg. Room 140
> Albuquerque, NM 87131
> (505) 272-4028
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 5, Issue 20
> ***************************************
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
<< Previous Message | Next Message >>