[Histonet] Safranine o staining and Problem in tissues
Can anyone give me the detailed procedure for safranine o staining for the
joints (ankle. tarsal and metatarsal joints in rats). I would like to know
a good decalcifying solution for the same and what is the duration of
bone to be kept in the decalcifier.
Secondly, of late I am facing peculiar problem now with my liver, kidney
and brain sections of late. These sections are cracking after the staining
(Mayer's H& E). It is something like parched earth appearance. This I am
noticing when the tissue s are coming to last xylenes. I donot see the
cracking before this step. This doesn't happen in all the slides. Could
you please suggest some solution for this. We are using chloroform for
clearing and histoplast from Shandon for infiltration. I am unable to
locate where exactly is the problem. I have tried changing the makes of
chloroform and xylenes used with out much help.
Dr. Balaji (M. V. Sc. Path)
Dept. Pre clinical safety evaluation,
Dr. Reddys Laboratories ltd. Bollaram Road,
Miyapur, Hyderabad, 500 049
Andhra Pradseh, INDIA
Tel: 23045439 - Ext.432.
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