[Histonet] RE: Histonet Digest, Vol 5, Issue 8
Hello,
I'd like to announce an immediate opening for a full-time histotechnician in the pathology department at the Wildlife Conservation Society located at the Bronx Zoo. We provide the diagnostic pathology services to one of the largest zoological collections in the country. Applications are being accepted until May 15, 2004 or until the position is filled. Please contact Ms. Tawanda Williams (Human Resources; email: twilliams@wcs.org) if you're interested in this exciting opportunity and in becoming part of our team!
Thanks for listening and we look forward to hearing from you.
D McAloose, VMD, Dipl ACVP
Head, Department of Pathology
Wildlife Conservation Society
Bronx, NY 10464
(phone) 718-220-7105
(fax) 718-220-7126
dmcaloose@wcs.org
-----Original Message-----
From: histonet-request@lists.utsouthwestern.edu
[mailto:histonet-request@lists.utsouthwestern.edu]
Sent: Tuesday, April 06, 2004 1:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 5, Issue 8
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Today's Topics:
1. RE: perfusion debate (Tamara Howard)
2. RE: RE: perfusion debate (Charles Scouten)
3. Microscopist position available, U of Richmond (Gary Radice)
4. (no subject) (Ant Swensson)
5. Region III Meeting (LaFriniere, Mike)
6. Adhesive for paraffin sections (Yin Ping Lee)
7. Analytical Imaging Station Version 6.0 (Wendy England)
8. RE: Region III Meeting (LaFriniere, Mike)
9. Re: schools (Lee & Peggy Wenk)
10. a problem about immunostaining for immunoglobulin heavy
chains (=?gb2312?q?=CA=E7=C7=ED=20=C9=F2?=)
11. a lot of stuff HELP OUT THERE!! (Jesus Ellin)
12. Re: Analytical Imaging Station Version 6.0 (Geoff McAuliffe)
13. Human myofibroblast markers (Mark Elliott)
14. (no subject) (Ronald E. Bremer)
15. porcine insulin (Stryker, S. (HLK))
16. Re: Sponge problems in processing (Steven E. Slap)
17. Ventana Tissue Processor (Vickroy, Jim)
18. Re: microwave antigen retrieval (Steven E. Slap)
19. solochrome stain (Myri37@aol.com)
20. RE: Sponge problems in processing
(Marshall Terry Dr, Consultant Histopathologist)
21. Re: re mirowave Ag retrieval (Steven E. Slap)
22. I need help! (María Teresa Domínguez)
23. Ronald Bremer/knife sharpener (Joyce Cline)
----------------------------------------------------------------------
Message: 1
Date: Mon, 5 Apr 2004 11:56:45 -0600 (MDT)
From: Tamara Howard
Subject: [Histonet] RE: perfusion debate
To: HistoNet
Message-ID:
Content-Type: TEXT/PLAIN; charset=US-ASCII
As far as the relationship of mm Hg to ml/min with a perfusion pump -
aren't the innner diameter and stiffness of the tubing (and the i.d. of
the cannula) going to influence the final pressure, too? I don't think
adjusting final pump speed alone is going to accurately duplicate the
"hanging bag" pressure. Can you check the literature and see what other
groups have reported and work from there?
|--------------------------------------------------|
Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
thoward@unm.edu
|--------------------------------------------------|
------------------------------
Message: 2
Date: Mon, 5 Apr 2004 13:24:14 -0500
From: "Charles Scouten"
Subject: RE: [Histonet] RE: perfusion debate
To: "Tamara Howard" , "HistoNet"
Message-ID:
Content-Type: text/plain; charset="iso-8859-1"
It depends on where the bottleneck is. If the cardiovascular system is the main source of resistance, it will feel all 300 mm Hg. If the tubing or needle is small, it will absorb the pressure drop, and the cardiovasucalar system will get much less than the gauge pressure the perfusion bottle was pumped up to. A mouse has much more cardiovascular resistance than a rat. A pig would have much much less.
The largest suitable needle and tubing (1/4" ID tubing), would insure that the animal was the main source of resistance if the flow was small (mouse or even rat), but larger animals would require larger tubing and needle.
Remember, the hanging bag pressure needs to be about 13 feet above the animal to provide a pressure that will disrupt the blood brain barrier. The correct flow rate to use depends greatly on animal size and condition. The correct pressure is constant, but needs to be higher than traditional gravity flow can typically provide. So it is better to control pressure directly, rather than flow rate.
For an inexpensive means of implementing this strategy, and getting no shrink perfusions, go to www.myneurolab.com, click products, click sacrifice instruments under the Histology header, click Perfusion One.
Cordially,
Charles W. Scouten, Ph.D.
myNeuroLab.com
5918 Evergreen Blvd.
St. Louis, MO 63134
Ph: 314 522 0300
FAX 314 522 0377
cwscouten@myneurolab.com
www.myneurolab.com
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tamara Howard
Sent: Monday, April 05, 2004 12:57 PM
To: HistoNet
Subject: [Histonet] RE: perfusion debate
As far as the relationship of mm Hg to ml/min with a perfusion pump -
aren't the innner diameter and stiffness of the tubing (and the i.d. of
the cannula) going to influence the final pressure, too? I don't think
adjusting final pump speed alone is going to accurately duplicate the
"hanging bag" pressure. Can you check the literature and see what other
groups have reported and work from there?
|--------------------------------------------------|
Tamara Howard
Department of Cell Biology and Physiology
University of New Mexico - Health Sciences Center
Albuquerque, NM 87131
thoward@unm.edu
|--------------------------------------------------|
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 3
Date: Mon, 5 Apr 2004 14:10:39 -0400
From: Gary Radice
Subject: [Histonet] Microscopist position available, U of Richmond
To: histonet@lists.utsouthwestern.edu
Message-ID: <8B456638-872C-11D8-B0A1-000393BC23F4@richmond.edu>
Content-Type: text/plain; charset=US-ASCII; format=flowed
Hello everyone,
Please forward if you know someone who might be interested.
Gary Radice
Director of Microscopy and Imaging
University of Richmond
The Department of Biology at this highly selective, private, primarily
undergraduate university invites applications for a Director of
Microscopy and Imaging. Duties including supervising operation of a new
biological imaging facility, including operation and routine
maintenance of a TEM, SEM, and laser scanning confocal microscope and
associated equipment. Teaching responsibilities include training and
supervising faculty and student users in research and classroom
activities and courses based on interests and department needs;
assisting faculty and students in experimental design, specimen
preparation, digital photography, and analysis of results.
Qualifications: MS or PhD degree and experience with light and electron
microscopy and digital imaging, strong interpersonal skills and desire
and ability to work well in a team environment. This is a continuing
appointment that is not eligible for tenure but may include faculty
status.
Applications including a letter of interest, CV, evidence of expertise
in microscopy, and three letters of recommendation should be sent to
Dr. Gary Radice, Department of Biology, University of Richmond,
Richmond VA 23173 (gradice@richmond.edu). Review of applications will
begin on April 23, 2004. The appointment is expected to begin June 1,
2004.
The University of Richmond is committed to increasing the diversity of
our faculty and strongly encourages applications from women and
minorities. For more information on the department, resources, and
teaching assignment, see (http://biology.richmond.edu/)
Gary P. Radice gradice@richmond.edu
Department of Biology 804-289-8107 (voice)
University of Richmond 804-289-8233 (FAX)
Richmond VA 23173 http://www.richmond.edu/~gradice
------------------------------
Message: 4
Date: Mon, 05 Apr 2004 11:28:13 -0700
From: "Ant Swensson"
Subject: [Histonet] (no subject)
To: histonet@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain
Hi. I'm interested in taking the HT exam at the end of this year.
I've been studying by myself, and working in a lab to get hands on
experience. I am curious, if I need to in the future, where the
schools that train for the exam are located in the USA?
Thank you,
Antoinette Swensson
Univeristy of Washington/Harborview Medical Center
Neuropathology
325 9th Ave MS 359-791
Seattle, WA 98104
(206) 731-3910
_________________________________________________________________
[1]Limited-time offer: Fast, reliable MSN 9 Dial-up Internet access
FREE for 2 months!
References
1. http://g.msn.com/8HMBENUS/2734??PS=
------------------------------
Message: 5
Date: Mon, 5 Apr 2004 13:29:06 -0400
From: "LaFriniere, Mike"
Subject: [Histonet] Region III Meeting
To:
Message-ID:
Content-Type: text/plain; charset="us-ascii"
The Region III Meeting is finalizing plans for their annual meeting this
year in Birmingham, Alabama. May 20th-24
The program can be found on the Alabama Society for Histotechnology
(ASH) web page. The web site can be found at www.timjday.com
and click on Region III, registration is
welcome through the website.
The hotel accommodations are at the beautiful Wynfrey attached to the
Galleria Mall in Birmingham. We ask that all participants register at
the Wynfrey to keep our meeting cost to a minimum! The negotiated rate
for the meeting is $125.00 per room, which normally is $199.00. This is
an upscale 4 star hotel! Please make your reservations at the Wynfrey as
soon as possible to obtain this rate as there is less than 20 days to
obtain the negotiated $125.00 per night rate!
We are demonstrating an excellent program to include more than 20
vendors and 14 excellent workshops offered from speakers within our
Region and expect over 100 attendees, this will hopefully help diminish
our debt from last years meeting in Puerto Rico. Your attendance at the
Wynfrey 1800-476-7006 is urgently requested to support Region III.
Please contact the hotel for reservations and state you are with the
Region III, Alabama Histology meeting!
Programs have been mailed out last week to all of Region III membership
as well as several State memberships!
Vendors are encouraged to also use the Wynfrey to assist with our
overall cost, in addition, there are a few spots left for Vendors who
have not yet registered to be at the meeting and can contact me directly
for additional information.
Michael LaFriniere
NSH Region III Director
423-495-6117
------------------------------
Message: 6
Date: Mon, 5 Apr 2004 16:35:44 -0700
From: Yin Ping Lee
Subject: [Histonet] Adhesive for paraffin sections
To: "'histonet@lists.utsouthwestern.edu'"
Message-ID: <6BAF4D075F07D411B30900508B94CBA0C1F55F@SERVER20>
Content-Type: text/plain; charset="iso-8859-1"
Hi, Leslie,
"Mount Quick Tissue Transfer Technique" is another alternative for your
case. This technique allows for the transfer of tissue sections from one
slide to another slide. (Therefore, the sections can be transferred to
charged slides and immuno-staining can be performed as per its usual
protocol.) This transfer technique is very simple to do; all you need is to
purchase the "Mount Quick" medium and follow the protocol. This medium is
distributed by Newcomer Supply. Tel: (608) 831-7888. Fax: (608)831-0866.
Yin Ping Lee
Histopathology Lab
BC Cancer Agency
Vancouver BC
------------------------------
Message: 7
Date: Mon, 05 Apr 2004 16:41:06 -0700
From: "Wendy England"
Subject: [Histonet] Analytical Imaging Station Version 6.0
To: histonet@pathology.swmed.edu
Message-ID:
Content-Type: text/plain; format=flowed
Hello,
I was asked to search info about above systems. But I couldn't find any
through my computer. Could anybody provide me the website I can look?
Any help will be highly appreciated!
Wendy
_________________________________________________________________
MSN Toolbar provides one-click access to Hotmail from any Web page - FREE
download! http://toolbar.msn.com/go/onm00200413ave/direct/01/
------------------------------
Message: 8
Date: Mon, 5 Apr 2004 18:26:11 -0400
From: "LaFriniere, Mike"
Subject: [Histonet] RE: Region III Meeting
To:
Message-ID:
Content-Type: text/plain; charset="us-ascii"
The Region III Meeting is finalizing plans for their annual meeting this
year in Birmingham, Alabama. May 20th-24
The program can be found on the Alabama Society for Histotechnology
(ASH) web page. The web site can be found at www.timjday.com
and click on Region III, registration is
welcome through the website.
The hotel accommodations are at the beautiful Wynfrey attached to the
Galleria Mall in Birmingham. We ask that all participants register at
the Wynfrey to keep our meeting cost to a minimum! The negotiated rate
for the meeting is $125.00 per room, which normally is $199.00. This is
an upscale 4 star hotel! Please make your reservations at the Wynfrey as
soon as possible to obtain this rate as there is less than 20 days to
obtain the negotiated $125.00 per night rate!
We are demonstrating an excellent program to include more than 20
vendors and 14 excellent workshops offered from speakers within our
Region and expect over 100 attendees, this will hopefully help diminish
our debt from last years meeting in Puerto Rico. Your attendance at the
Wynfrey 1800-476-7006 is urgently requested to support Region III.
Please contact the hotel for reservations and state you are with the
Region III, Alabama Histology meeting!
Programs have been mailed out last week to all of Region III membership
as well as several State memberships!
Vendors are encouraged to also use the Wynfrey to assist with our
overall cost, in addition, there are a few spots left for Vendors who
have not yet registered to be at the meeting and can contact me directly
for additional information.
Michael LaFriniere
NSH Region III Director
423-495-6117
------------------------------
Message: 9
Date: Tue, 6 Apr 2004 05:07:46 -0400
From: "Lee & Peggy Wenk"
Subject: Re: [Histonet] schools
To: "Ant Swensson" ,
Message-ID: <002b01c41bb6$a103dfc0$b834d445@domainnotset.invalid>
Content-Type: text/plain; charset="iso-8859-1"
The accrediting agency for lab schools is the National Accrediting Agency
for Clinical Laboratory Schools (NAACLS).
To find lab programs, including HT and HTL, go to
http://www.naacls.org
On the left side, click on "find a program"
Then, enter the type of program (HT or HTL, for exam), and the state (or
search for all states).
(There are 27 HT and 2 HTL = 29 accredited programs in the US. Up from about
21 a couple of years ago. Still could use a lot more, in my opinion.)
Peggy A. Wenk, HTL(ASCP)SLS
Program Director
School of Histotechnologists
School of Histologic Technicians
William Beaumont Hospital
Royal Oak, MI 48073
----- Original Message -----
From: "Ant Swensson"
To:
Sent: Monday, April 05, 2004 2:28 PM
Subject: [Histonet] (no subject)
>
> Hi. I'm interested in taking the HT exam at the end of this year.
> I've been studying by myself, and working in a lab to get hands on
> experience. I am curious, if I need to in the future, where the
> schools that train for the exam are located in the USA?
>
> Thank you,
>
> Antoinette Swensson
>
> Univeristy of Washington/Harborview Medical Center
> Neuropathology
> 325 9th Ave MS 359-791
> Seattle, WA 98104
> (206) 731-3910
> _________________________________________________________________
>
> [1]Limited-time offer: Fast, reliable MSN 9 Dial-up Internet access
> FREE for 2 months!
>
> References
>
> 1. http://g.msn.com/8HMBENUS/2734??PS=
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 10
Date: Tue, 6 Apr 2004 20:22:03 +0800 (CST)
From: =?gb2312?q?=CA=E7=C7=ED=20=C9=F2?=
Subject: [Histonet] a problem about immunostaining for immunoglobulin
heavy chains
To: histonet@lists.utsouthwestern.edu
Message-ID: <20040406122203.67972.qmail@web15207.mail.bjs.yahoo.com>
Content-Type: text/plain; charset=gb2312
Hello,everybody,
I am intrested in immunostaining for immunoglobulin heavy chains on renal biopsy specimen ,but I have no idea about which antibodies are appropriate.Would anybody be kind enough to share me with you experience?
Thank You very much.
Best regards!
Shuqiong Shen
Jinling Hospital
Nanjing 210002
P.R.China
---------------------------------
Do You Yahoo!?
»ÝÆÕTTÓÎÏ·¾ç£¬ÍæÓÎÏ·£¬Öдó=BD±£¡
------------------------------
Message: 11
Date: Tue, 06 Apr 2004 05:36:11 -0700
From: "Jesus Ellin"
Subject: [Histonet] a lot of stuff HELP OUT THERE!!
To: histonet@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; charset=us-ascii
Hello everyone thought that I would ask for some help here,, Currently our lab is getting a hugh over haul on everthing and I need to find out some information well were do I begin,
First off our lab went live with Tamtron this past year and it has been different to say the least, but one of the ideas we are pondering is using the image module to caputre gross instead of gross dictation, only on small bx that do not require anything other than transfering it to the cassette and snapping a picture. Was wondering if anyone out there is doing this and also if they do have Tamtron how do they like the imaging module.
The next item we are looking at is this processor that can process tissue in 1 hr and is any one out there doing this? This is a huge step, since we are part of the traditional way of processing over night and doing the embedding the next day. SO if anyone is attempting this please feel free to give me input.
The last thing that our lab is going to under take is allow tech that are not certified and do not have 60 hrs of college to do gross,the standard is set forth in NACCLS that a person must have a minimal amount of education to do this. But our pathologist called the CAP and said that they can do transfer of specimens that can give color, size, dimension. This is were the idea of photographing specimens came amout.
ANY help would greatly be appreciated.
Jesus Ellin HTL
Yuma Regional Medical Center
------------------------------
Message: 12
Date: Tue, 06 Apr 2004 10:09:23 -0700
From: Geoff McAuliffe
Subject: Re: [Histonet] Analytical Imaging Station Version 6.0
To: Wendy England
Cc: histonet@pathology.swmed.edu
Message-ID: <4072E443.1090405@umdnj.edu>
Content-Type: text/plain; format=flowed; charset=windows-1252
Ask the manufacturer of the system.
If you are looking to purchase a new image analysis system Nikon, Zeiss,
Olympus etc all make such systems. In addition, there are many vendors
who will build a system to meet your current and future needs.
Geoff
Wendy England wrote:
> Hello,
> I was asked to search info about above systems. But I couldn't find
> any through my computer. Could anybody provide me the website I can look?
>
> Any help will be highly appreciated!
>
> Wendy
>
> _________________________________________________________________
> MSN Toolbar provides one-click access to Hotmail from any Web page -
> FREE download! http://toolbar.msn.com/go/onm00200413ave/direct/01/
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
**********************************************
------------------------------
Message: 13
Date: Tue, 06 Apr 2004 07:30:40 -0700
From: "Mark Elliott"
Subject: [Histonet] Human myofibroblast markers
To:
Message-ID:
Content-Type: text/plain; charset=US-ASCII
Was wondering what everyone is using for labelling myofibroblasts in
human tissue? Preferably something that works in FFPE tissues, but
frozen is OK.
Thanks
Mark
W. Mark Elliott, PhD
Research Associate,
Interim Director, Histology Core Facility
James Hogg iCAPTURE Centre for Cardiovascular and Pulmonary Research
Room 166, St. Paul's Hospital
1081 Burrard Street
Vancouver, BC
Canada V6Z 1Y6
------------------------------
Message: 14
Date: Tue, 06 Apr 2004 10:34:18 -0400
From: "Ronald E. Bremer"
Subject: [Histonet] (no subject)
To: histonet@lists.utsouthwestern.edu
Message-ID: <4072BFEA.3010808@acpub.duke.edu>
Content-Type: text/plain; charset=us-ascii; format=flowed
We are intertested in purchasing a used microtome knife sharpener. I was
hoping for some advice. I've noted a few American Optical models for
sale for what seems a rather cheap price. I think Leica picked them up
or is the same company. Are these shapreners difficult to get parts for?
I'm also interested in comments on "Hacker knife sharpeners" Even new
they seem rather inexpensive compared to other models.
We could go new if the price was right and have GSA money if anybody
could point me in the right direction. Unfortunately the right price is
less than $1000, so I am not hopeful on a new model.
Ron
------------------------------
Message: 15
Date: Tue, 6 Apr 2004 17:04:47 +0200
From: "Stryker, S. (HLK)"
Subject: [Histonet] porcine insulin
To: "'histonet@lists.utsouthwestern.edu'"
Message-ID:
Content-Type: text/plain; charset="iso-8859-1"
Hello everybody,
I'm hoping someone can give me any suggestions on how to detect insulin on a
(paraffin embedded formalin-fixed or frozen) porcine section using the
following antibodies:
- primary antibody: Guinea Pig anti-insulin (Dako)
- secondary antibody: Swine anti-Rabbit (Dako)
According to the DAKO catalogous the anti-Rabbit should work in this combo.
I have tried it over and over again, using different protocols, but somehow
I just can't get the job done...
Thanks in advance!
Samantha
s.stryker@lumc.nl
Leiden University Medical Centre
------------------------------
Message: 16
Date: Tue, 6 Apr 2004 11:13:41 -0400
From: "Steven E. Slap"
Subject: Re: [Histonet] Sponge problems in processing
To: Gayle Callis , "Gudrun Lang"
, Histonet@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; charset="us-ascii" ; format="flowed"
Hi HistoNetters
I agree with Gayle, and the other posters who pointed out that
sponges carry over 1ml of fluid per sponge (so, with 100 cassettes,
200 sponges, that's a lot of carryover).
I have had a lot of success with the biopsy cassettes which Gayle
refers to, both in microwaves and in conventional tissue processors.
You can request samples from Lab Storage Systems in St. Louis by
phone at (800) 345-4167 or by e-mailing Rita Lovshe at
rcl@labstore.com.
best regards,
Steven Slap
At 10:20 AM -0700 4/1/04, Gayle Callis wrote:
>To: "Gudrun Lang" , Histonet@lists.utsouthwestern.edu
>From: Gayle Callis
>Cc:
>Subject: [Histonet] Sponge problems in processing
>
>Gudrun,
>
>We no longer use sponges, prefer to place tissues in tissue embedding bags
>(Fisher) which look like tea bags or the little nylon bags (not as easy to
>handle, but thinner than sponges) and a tidge stiffer than tea bags.
>Sponges can cause artifacts in your tissues, looking like triangular holes
>in section. This was published by Freida Carson in Journal of
>Histotechnology, 1980's. Using tea bags may not be as fast during
>embedding, but speed there is a trade off for having to reprocess important
>tissue samples, ho hum tedious and time consuming while patient waits.
>
>You analysis of problem sound correct with a poor exchange of solvents
>through sponges. If you pack cassettes super tight in basket/cassette
>holder inside processor you can impede solvent flow or if sponges are
>crammed too tight agaist tissue then lid smashed down on tissue/sponge
>sandwich - this is like having too thick a tissue in a cassette.
>
>There are some clever biopsy cassettes with a folding, fine mesh inserts
>fitting inside a cassette. These may be worth a try to avoid sponges. I
>think they are available through Fisher or some other company who could
>provide free samples. It will be interesting to see peoples testimonials
>on using these.
>
>There was Histonet discussion on these sponges way back in time - it may
>pay to do a search for those messages in Histonet Archives.
------------------------------
Message: 17
Date: Tue, 6 Apr 2004 09:57:30 -0500
From: "Vickroy, Jim"
Subject: [Histonet] Ventana Tissue Processor
To: "Histonet \(E-mail\)"
Message-ID:
Content-Type: text/plain
We had a processing run today that looks like the tissue was cooked especially the small GI specimens. We have a Ventana Renissance Tissue Processor, which they no long sell. Has anyone every had a problem similar to this, and is there any correlation to problems with the tissue processor? My first thoughts are that maybe the chemistry on the machine was switched, but I can't find any glaring problems. Thanks for your help
James R. Vickroy BS, HT (ASCP)
Technical Supervisor, Surgical Pathology
788-4046
vickroy.jim@mhsil.com
This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited.
------------------------------
Message: 18
Date: Tue, 6 Apr 2004 11:19:33 -0400
From: "Steven E. Slap"
Subject: Re: [Histonet] microwave antigen retrieval
To: Gayle Callis ,
Histonet@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"
Hi HistoNetters
Successful antigen retrieval depends on accurate
control of three factors: time, temperature and
pH. Any instrument which will produce this
accuracy in a reproducible way will be effective.
A microwave has been shown to work well over a
wide range of antigens, where other methods have
required more aggressive treatment. In some
cases, pressure is needed to allow for
temperatures above 98°C. I can get most antigens
to "work" with pH 6.0 citrate buffer at 110°C in
a 3-5 minute microwave method using the
Hacker/Milestone microwave.
best regards,
Steven Slap
Microwave Consultant
------------------------------
Message: 19
Date: Tue, 06 Apr 2004 11:18:31 -0400
From: Myri37@aol.com
Subject: [Histonet] solochrome stain
To: histonet@pathology.swmed.edu
Message-ID: <64E5A68B.38CAA850.0005167B@aol.com>
Content-Type: text/plain; charset=iso-8859-1
hello
i have prepared solochrome stain following this:
1 g of calcon (solochrome)
2 ml acetic acid glacial
98 ml distilled water
and let it for 3 months before use
i tried this stain on MMA sections of thin reconstructed and mineralized tissue,
i put section of 50um in this stain for 1 hour, i didn't see anything stained on hot plate or not
could any one help me ? is the stain not good ? or something else ?
thank you very much for your help
Myriam
------------------------------
Message: 20
Date: Tue, 6 Apr 2004 16:20:47 +0100
From: "Marshall Terry Dr, Consultant Histopathologist"
Subject: RE: [Histonet] Sponge problems in processing
To: "Steven E. Slap" , "Gayle Callis"
, "Gudrun Lang" ,
Message-ID:
Content-Type: text/plain; charset="iso-8859-1"
OK. 200 ml. of liquid retained, with 100 blocks.
However, with a vacuum processor, is that not all "sucked away"?
BTW, should it sound otherwise, I hate the things (but am stuck with them).
Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
Consultant Pathologist
Rotherham General Hospital
South Yorkshire
England
terry.marshall@rothgen.nhs.uk
-----Original Message-----
From: Steven E. Slap [mailto:siksik03@comcast.net]
Sent: 06 April 2004 16:14
To: Gayle Callis; Gudrun Lang; Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Sponge problems in processing
Hi HistoNetters
I agree with Gayle, and the other posters who pointed out that
sponges carry over 1ml of fluid per sponge (so, with 100 cassettes,
200 sponges, that's a lot of carryover).
I have had a lot of success with the biopsy cassettes which Gayle
refers to, both in microwaves and in conventional tissue processors.
You can request samples from Lab Storage Systems in St. Louis by
phone at (800) 345-4167 or by e-mailing Rita Lovshe at
rcl@labstore.com.
best regards,
Steven Slap
At 10:20 AM -0700 4/1/04, Gayle Callis wrote:
>To: "Gudrun Lang" , Histonet@lists.utsouthwestern.edu
>From: Gayle Callis
>Cc:
>Subject: [Histonet] Sponge problems in processing
>
>Gudrun,
>
>We no longer use sponges, prefer to place tissues in tissue embedding bags
>(Fisher) which look like tea bags or the little nylon bags (not as easy to
>handle, but thinner than sponges) and a tidge stiffer than tea bags.
>Sponges can cause artifacts in your tissues, looking like triangular holes
>in section. This was published by Freida Carson in Journal of
>Histotechnology, 1980's. Using tea bags may not be as fast during
>embedding, but speed there is a trade off for having to reprocess important
>tissue samples, ho hum tedious and time consuming while patient waits.
>
>You analysis of problem sound correct with a poor exchange of solvents
>through sponges. If you pack cassettes super tight in basket/cassette
>holder inside processor you can impede solvent flow or if sponges are
>crammed too tight agaist tissue then lid smashed down on tissue/sponge
>sandwich - this is like having too thick a tissue in a cassette.
>
>There are some clever biopsy cassettes with a folding, fine mesh inserts
>fitting inside a cassette. These may be worth a try to avoid sponges. I
>think they are available through Fisher or some other company who could
>provide free samples. It will be interesting to see peoples testimonials
>on using these.
>
>There was Histonet discussion on these sponges way back in time - it may
>pay to do a search for those messages in Histonet Archives.
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------------------------------
Message: 21
Date: Tue, 6 Apr 2004 11:34:30 -0400
From: "Steven E. Slap"
Subject: Re: [Histonet] re mirowave Ag retrieval
To: "Carl" ,
Message-ID:
Content-Type: text/plain; charset="us-ascii" ; format="flowed"
Hi HistoNetters
The Nordicware microwave pressure cooker is available at a deep
discount from the Yahoo store:
Nordicware Tender Cooker/Microwave Pressure Cooker 62104
shop.store.yahoo.com/dotcoms/tecoprcobyno.html
However, this device does not allow for temperature control. Let the
buyer beware...
best regards,
Steven Slap
At 8:36 PM +0100 4/1/04, Carl wrote:
>
>Well...I use a microwave pressure cooker! I understand that one can
>buy this in USA Walmarts ( be interested to know the price of it in
>the USA). I buy them in UK from Biogenex. Very reliable and
>consistent.. Started on Coplins jars and , VERY rapidly moved to
>rice staemers, pressure cookers...then microwave pressure cookers. I
>look back in no anger.
------------------------------
Message: 22
Date: Tue, 06 Apr 2004 13:44:57 -0300
From: "María Teresa Domínguez"
Subject: [Histonet] I need help!
To: Histonet@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain
Hi Histonetters!
In our lab we have received Methilyc Alcohol
which we use in the tissues prossessor, but this time we don't know
the Quality of it due to the quantity we have received doesn't have
any label with any descripcion about it's features.
I wonder if there any quality Control about Methilyc Alcohol. What I
need to know if this Alcohol is 100% methilyc.
I'll appreciate all you can advice me.
H.T. Maria Teresa Dominguez
Rio Grande, Zonal Hospital,
Anatomic Pathology,
Tierra del Fuego, Argentina.
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------------------------------
Message: 23
Date: Tue, 6 Apr 2004 12:57:15 -0400
From: "Joyce Cline"
Subject: [Histonet] Ronald Bremer/knife sharpener
To: "Histonet"
Message-ID: <002d01c41bf8$3a14d660$1d2a14ac@wchsys.org>
Content-Type: text/plain;charset="iso-8859-1"
I have a Hacker knife sharpener for sale, it works perfectly and I even have the video that shows how to work it. I have used glass sharpeners in the past and the Hacker beats any sharpener for doing the steel knives.
We are using disposables now so I have no need for the Hacker. You can email me directly if you would like.
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End of Histonet Digest, Vol 5, Issue 8
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