Hi Histonetters,

I am looking for a good tissue processing schedule for mouse pancreas. I
am currently using the following schedule and having trouble with
sectioning the pancreas, the H&E's look cracked and parched as if my
water bath is too hot. The temperature on my water bath is 42-43 degrees
Celsius, I have a thermometer also that I check the temp with. I use
Tissue Prep paraffin for infiltration and embedding. 

Fixed by the researcher in 4% PFA 24 hours rinsed in distilled water 5'
x 3. 

70% Alcohol- 30 min.
Prosoft- 1 hour
Prosoft- 1 hour
Prosoft- 1 hour
Prosoft- 1 hour
Prosoft- 1 hour
Prosoft- 1 hour 30 min.
Clearite 3- 1 hour 15 min.
Clearite 3- 1 hour 15 min.
Clearite 3- 1 hour 15 min.
Paraffin- 1 hour
Paraffin- 1 hour
Paraffin- 1 hour
Paraffin- 1 hour


Thanks in advance!


Debby Grant
Research Technician II
Histology Core Facility
Stowers Institute for Medical Research
1000 E. 50th Street
Kansas City, MO 64110
drg@stowers-institute.org
 

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