[Histonet] Melanin Bleach & IHC

From:"Lisa Black"

Hello All,
 Whenever we bleach a slide prior to IHC staining, the end result is
sub-optimal.    Our protocol is to use 0.5% potassium permanganate and
then oxalic acid for the bleach then to proceed with the automated IHC
stain using Ventana's DAB I-View Kit.   Most of the tissue comes off the
slide and the remaining tissue has poor morphology.  Does anyone have
good advice for me?  
Thanks in advance,
Lisa Black
Carilion Consolidated Lab
Roanoke, VA

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