[Histonet] Lectin IHC

From:"Amrit Samra"

I am doing lectin staining for frozen guinea pig tissues. I have tried
different fixitives; bouin, 10% formalin, acetone, ethanol, B5 and
different buffers; TBS, PBS and lectin buffer. I am still not able to
get a positive staining for my known positive. The last trial I did was
fixing in bouin and washing with TBS.
any help would be greatly appreciated.


Amrit Samra
Histology Lab,the iCAPTURE Centre
St.Paul's Hospital 
1081 Burrard Street,Vancouver,BC
Canada V6Z 1Y6
Phone: 604-682-2344 extension 62703

Histonet mailing list

<< Previous Message | Next Message >>