[Histonet] Decal question
I'm working on a trial with intra-tibial injections of tumors into mice.
Whole (skin and muscle trimmed) legs were fixed for about a month in 10%
NBF. I used Surgipath's Decal I to decalcify (took about 6 hours - end
point determined by cutting through femur with scalpel). Paraffin
sections cut nicely - I used a 48C waterbath to ensure no wrinkles, picked
up on positively charged slides. I let them air dry overnight, then 60C
for an hour prior to H+E staining. I'm not happy. The tumors and
cortical bone are lifting and falling off the slides. Any suggestions?
I've been reading old posts about EDTA decalcification - since hindsight
is 20/20 - would that have been a better choice?? Boo hoo.
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