[Histonet] BCL2 High pH retrieval
Try High pH antigen retrieval (pH 10.0). The steamer for 1/2 hour
should be fine. I can't avouch for the detection kit you're stuck using
with a Ventana stainer, but it should work. I would suggest a non biotin
detection kit like Envision from DAKO or Mach3 from Biocare (I'm sure there
are others). It seems high pH retrieval increases endogenous biotin.
At 09:20 PM 4/8/2004, you wrote:
>Date: Fri, 9 Apr 2004 18:51:44 -0400
>From: "Derek & Lynda Leopold"
>Subject: [Histonet] bcl-2 on Nexes
>Content-Type: text/plain; charset="iso-8859-1"
>We're having some trouble getting our tonsil control to come up for
>bcl-2. After searching the archives and asking around, we are going to
>try citrate buffer (Declere) and a hot steamer for 1 hour. We've got the
>Nexes IHC. Anyone else have any tips for us?
>Can anyone comment on the need/non-need for DAB?
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