FW: [Histonet] tissue processing and sponges

From:"S Ladd"

I forgot to copy this to the list yesterday.

-----Original Message-----
From: S Ladd [mailto:sladd@hsc.usf.edu]
Sent: Thursday, April 01, 2004 12:24 PM
To: Gudrun Lang
Subject: RE: [Histonet] tissue processing

This very same thing happenned to us 2 weeks ago. We loaded approximately
200 cassettes on the new VIP and about 75% of them had sponges.
Approximately 50 of the blocks were not processed at all. The 50 blocks were
all in the same area of the basket and they were all in the bottom basket
not the top! The size of the tissue did not correlate with the how well the
specimens were processed either; some big excisions processed and some
little shaves did not. We checked all of the solutions and we even checked
the formalin vials from the unprocessed cases and everything was fine. It
was a complete and total mystery. Later someone noticed that the agitation,
P/V AND temperature had been turned off on all of the stations on the
program that we had run. Having a lot of sponges probably compounded the
problem. So....try checking the P/V, agitation and temperature. What a
frustrating experience! I never thought in a million years that someone else
would have had the very same problem!
University of South Florida

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Gudrun
Sent: Thursday, April 01, 2004 5:10 AM
To: Histonetliste
Subject: [Histonet] tissue processing

We have a current problem with our routine tissue processing in the VIP. We
loaded 200 capsules in the container. This time we had the half of the
capsules filled with biopsy-sponges. Usually they are only about 30% of all.
We do over night processing.
The tissue surface was something like creamy and in the trimmed block the
tissue looked wet. I was not able to get a good section (only holes in
paraffin). But not all blocks were like this, most of the small biopsies
worked well.
My suggestion is, that the great amount of sponges is responsible for the
underprocessing. I think the tissue was'nt dehydratet enough and water was
taken over from one step to the next.

Did anybody have the same experience? Please give me some input on this

thanks in advance
Gudrun Lang
Akh Linz, Austria

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