I have been having a problem with my microwave technique for the past few days. I have been using the same protocol for months and out of the blue two days ago, the tissue looks fried. The protocol is as follows:
4 minutes in preserve at 55 degrees
4 minutes in p3 (alcohol blend) at 67 degrees
4 minutes in isopropol alcohol at 74 degrees
4 minutes in paraffin at 74 degrees
I have been thinking of trying a different fixative, but not so sure.
Some of the problems that we have had the past couple of days are completely opposite of each other, but we will get very dried out tissue, fried looking tissue, or tissue that doesn't look processed or fixed. The protocol above is for our small biopsies < 3mm.
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