ask for a protocol for CD3 or CD79a on previously stained slides....
I'm asking for a protocol for applying CD1a, CD3 or CD79a on
previously stained slides, especially by Romanoswsky type stains.
I'm doing diagnostic cytology in dogs and cats, and I would
like to to set up a protocol for previously stained slides by
Wright, Giemsa, or May Grunwald Giemsa stains, because, there are
increasing tumor cases where we can only obtain a few stained cytologic
smears and no mass excised for confirmation! Pet owners avoid surgery frequently
for finacial constrains , poor prognosis or lack of information for
BUt NO tentative diagnosis without histologic or
immunohistochemical confirmation bear any meaning in
clinical oncology!! In fact, I have attemted to apply some
antigens on Wright stained slides and got some successful
results, but as for CD antigens such as CD1a, 3, or 79a I have had no
idea! I have used antigen retrieval system using citrate
buffer,pH6.0 after smear trasfer using Mount Quick to adhesive
slides, but the results went unsuccesful. Any help in any
form would be appreciated. If I have ever tried awful things with CD antigens so
far, please let me hear No way, or if anyone with experiances
immunoCYTOchemistry on Wright stained slides using CD antigens, give me tips for
the procedures. Any cautions or advice, or information based on literature would
be great help to me.
A protocol for applying CD antigens on Wright stained
Thank you in advance.
Ul Soo, Choi
DVM, graduate student
Dept of clinical pathology, Veterinary college
Seoul Nat'l Univ, South Korea
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