Secondary Antibody and Streptavidin/Peroxidase incubation time

From:Fernando Capela e Silva

In general immunohistochemistry protocol some companies refered 10-15  min
as incubation time for Secondary Antibody and for Streptavidin/Peroxidase.
There is any problem if this time is of 30-60 min?

With compliments

* * * * * * * * * * * * * * * * * * * * * * * * *
Fernando Capela e Silva
Laborat#243#rio de Biologia da Conserva#231##227#o
Departamento de Biologia
Universidade de ...vora
Apartado 94
7002-554 ...vora
PORTUGAL

Phone: +351-266 760 800
Fax: +351-266 711 231
Email: fcs@uevora.pt

http://evunix.uevora.pt/~fcs/FernandoCapelaSilva.htm





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