I would think the concentration of secondary would be the key.  I
normally incubate for 1 hr at RT.  When I'm rushed, 45 min works, too, at
the dilution I use.



John Carroll Dennis
Anatomy, Physiology, and Pharmacology
109 Greene Hall
Auburn University, AL  36849


On Tue, 29 Apr 2003, Fernando Capela e Silva wrote:

> In general immunohistochemistry protocol some companies refered 10-15  min
> as incubation time for Secondary Antibody and for Streptavidin/Peroxidase.
> There is any problem if this time is of 30-60 min?
>
> With compliments
>
> * * * * * * * * * * * * * * * * * * * * * * * * *
> Fernando Capela e Silva
> Laborat#243#rio de Biologia da Conserva#231##227#o
> Departamento de Biologia
> Universidade de ...vora
> Apartado 94
> 7002-554 ...vora
> PORTUGAL
>
> Phone: +351-266 760 800
> Fax: +351-266 711 231
> Email: fcs@uevora.pt
>
> http://evunix.uevora.pt/~fcs/FernandoCapelaSilva.htm
>
>
>