Re: Secondary Antibody and Streptavidin/Peroxidase incubation time
I would think the concentration of secondary would be the key. I
normally incubate for 1 hr at RT. When I'm rushed, 45 min works, too, at
the dilution I use.
John Carroll Dennis
Anatomy, Physiology, and Pharmacology
109 Greene Hall
Auburn University, AL 36849
On Tue, 29 Apr 2003, Fernando Capela e Silva wrote:
> In general immunohistochemistry protocol some companies refered 10-15 min
> as incubation time for Secondary Antibody and for Streptavidin/Peroxidase.
> There is any problem if this time is of 30-60 min?
> With compliments
> * * * * * * * * * * * * * * * * * * * * * * * * *
> Fernando Capela e Silva
> Laborat#243#rio de Biologia da Conserva#231##227#o
> Departamento de Biologia
> Universidade de ...vora
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> 7002-554 ...vora
> Phone: +351-266 760 800
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