Re: Myelin processing

From:Geoff McAuliffe

The solvents used in paraffin processing, alcohols and clearing agents, 
will dissolve the myelin from your speciimens. You might want to try 
frozen sections instead.

Geoff

xchen@neurology.bsd.uchicago.edu wrote:

>Does anybody there know of the paraffin tissue processing protocol for mouse
>CNS and PNS using Leica TP1020 automatic tissue processor? We are having a
>hard time to preserve the myelin structure of mouse tissue. Any input is
>highly appreciated.
>
>Xiangjun Chen
>University of Chicago
>xchen@neurology.bsd.uchicago.edu
>
>  
>

-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff@umdnj.edu
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