Marge, from the transport medium we rinse in sucrose for 15 mins, take the tissue out wrap it in foil and freeze it -80 deg C, it keeps indefinitely until we cut it.
Mike

-----Original Message-----
From: marjorie lehman [mailto:Marjorie.Lehman@unilever.com]
Sent: Thursday, 17 April 2003 3:51 AM
To: histonet
Subject: sucrose/cryo

Hi Netters,
I've searched archives but couldn't anything on how long it is OK to leave
tissue in sucrose. So the question is, could I store tissue in sucrose for 2
days? Refrigerate? or would I wreck the tissue?
I'm going to get a lot of tissue very late Friday afternoon and if I have to
snap freeze it I will be here until Saturday! I'm not usually this lazy, but
it's been a week of Mondays, and I am realy, really tired!
Marge