RE: bone

From:Gerard Spoelstra

You can purchase this book at .com
At 08:02 AM 24/04/03 -0500, you wrote:
>Where cn I purchase this book
>-----Original Message-----
>From: Gerard Spoelstra []
>Sent: Thursday, April 24, 2003 4:06 AM
>Subject: Re: bone
>You need to melt down the block and reverse-process it ,Gayle Callis
>describes this  in her chapter on bone in the 5th edition of Theory and
>practice if Histological Techniques.
>Take the bone back through a couple of changes of xylene a few hours in
>each and then through two changes of absolute alcohol a few hours in each
>to remove any residual water. Most problems associated with the processing
>of bone are caused by inadequate dehydration. We leave our bone in 1%
>fabric softener made up in tap water after decalcification and prior to
>processing and it processes using normally with our other tissue on an
>overnight schedule.
>Gerard Spoelstra
>Medical Scientist
>Veterinary Histology
>Murdoch University
>   At 04:42 PM 23/04/03 -0500, you wrote:
> >


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