Atoska,

I would just remelt your bone block and put it back into paraffin into a
vacuum oven at 15 lbs. of pressure either for a few hours or overnight
depending on the size. Bone is very dense so it takes much, much longer to
infiltrate so the longer in paraffin the easier it is to cut. (also have to
make sure it was in xylenes long enough to clear)
Good luck.

Peggy DiCarlo HT/MLT (ASCP)



-----Original Message-----
From: Atoska S. Gentry [mailto:gentras@vetmed.auburn.edu]
Sent: Wednesday, April 23, 2003 17:43
To: histonet
Subject: bone



Does anyone have a tried and proven remedy for retrieving sufficient 
sections from decalcified bone that yielded inadequate infiltration with 
paraffin during processing? It is just crumbling on all attempts to 
section. Your prompt replies will be very much appreciated. Thanks! Atoska


Atoska S. Gentry B.S., HT(ASCP)
Research Assistant III
Scott-Ritchey Research Center
College of Veterinary Medicine
Auburn University, AL  36849
Phone# (334)844-5579  Fax# (334)844-5850