RE: ask for a protocol for CD3 or CD79a on previously stained slides....
Dear Ul Soo,
As those antigens are probably heavily damaged by staining and storing the
stained slides at room temperature (??), I am afraid CD1a, CD79a and CD3
IHC staining is going to be extremely difficult, or perhaps impossible. The
only change you have is trying the tyramide amplification IHC procedure. A
kit system by Perkin & Elmer (NEL-700) provides the highest amplification
possibilities for IHC right now. Lots of success.
Chris van der Loos
Dept. of Cardiovascular Pathology
Academical Medical Center
Amsterdam - The Netherlands
Ul Soo wrote:
>Date: 21 Apr 2003 12:30:23 -0600
>From: Ul Soo
>Subject: ask for a protocol for CD3 or CD79a on previously stained slides....
>I'm asking for a protocol for applying CD1a, CD3 or CD79a on previously
>stained slides, especially by Romanoswsky type stains.
>I'm doing diagnostic cytology in dogs and cats, and I would like to to set up
>a protocol for previously stained slides by Wright, Giemsa, or May Grunwald
>Giemsa stains, because, there are increasing tumor cases where we can only
>obtain a few stained cytologic smears and no mass excised for confirmation!
>Pet owners avoid surgery frequently for finacial constrains , poor prognosis
>or lack of information for successful treatment.
>BUt NO tentative diagnosis without histologic or immunohistochemical
>confirmation bear any meaning in clinical oncology!! In fact, I have
>to apply some antigens on Wright stained slides and got some successful
>results, but as for CD antigens such as CD1a, 3, or 79a I have had no idea! I
>have used antigen retrieval system using citrate buffer,pH6.0 after smear
>trasfer using Mount Quick to adhesive slides, but the results went
>unsuccesful. Any help in any form would be appreciated. If I have ever tried
>awful things with CD antigens so far, please let me hear No way, or if anyone
>with experiances immunoCYTOchemistry on Wright stained slides using CD
>antigens, give me tips for the procedures. Any cautions or advice, or
>information based on literature would be great help to me.
>A protocol for applying CD antigens on Wright stained cytologic smears!
>Thank you in advance.
>Ul Soo, Choi
>DVM, graduate student
>Dept of clinical pathology, Veterinary college
>Seoul Nat'l Univ, South Korea
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