1. Pararosaniline hydrochloride (CI No. 42500)                50 mg
2. Dissolve in boiling water                                            100 mL
3. Filter through Whatman No. 1 filter paper
4. Add sodium thiosulfate crystal (Na2S2O3 . 5H2O)        24 gm
5. Add concentrated HCl                                                1 mL
6. Stir until solution is crystal clear and slightly pink and sulphur precipitate is pink to white.  About 1-2 hours required.
7. Refrigerate for 4 hours; overnight is preferable.  Filter before use and store in the dark.  Solution is completely colorless.

-----Original Message-----
From: Tony Henwood [mailto:AnthonyH@chw.edu.au]
Sent: Wednesday, April 16, 2003 6:44 PM
To: 'Angela Hammond'; 'HistoNet Server'
Subject: RE: Schiff's Reagent

Angela,

Have you tried Lillee's Cold Schiff method - no heating required.

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html

-----Original Message-----
From: Angela Hammond [mailto:hammond1@iprimus.com.au]
Sent: Wednesday, 16 April 2003 21:24
To: 'HistoNet Server'
Subject: Schiff's Reagent

I am curious to know why it is important to cool Schiffs reagent to 50degC before adding the potassium metabisulphate.  I have seen this in a number of methods. Does anyone have an explanation for this? ie. Is that temperature really that important? Thankyou.

Angela Hammond
Histology Dept.
Clinpath Laboratories
Adelaide, South Australia



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