RE: Schiff's Reagent

From:Tony Henwood

RE: Schiff's Reagent

Angela,

Have you tried Lillee's Cold Schiff method - no heating required.

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html



-----Original Message-----
From: Angela Hammond [mailto:hammond1@iprimus.com.au]
Sent: Wednesday, 16 April 2003 21:24
To: 'HistoNet Server'
Subject: Schiff's Reagent


I am curious to know why it is important to cool Schiffs reagent to 50degC before adding the potassium metabisulphate.  I have seen this in a number of methods. Does anyone have an explanation for this? ie. Is that temperature really that important? Thankyou.

Angela Hammond
Histology Dept.
Clinpath Laboratories
Adelaide, South Australia



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