RE: Schiff's Reagent

From:"Marshall Terry Dr, Consultant Histopathologist"

There are plenty of pink floors to attest to the importance of temperature:-)

Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
 Consultant Pathologist
 Rotherham General Hospital
 South Yorkshire

-----Original Message-----
From: Angela Hammond []
Sent: 16 April 2003 12:24
To: 'HistoNet Server'
Subject: Schiff's Reagent

I am curious to know why it is important to cool Schiffs reagent to 50degC before adding the potassium metabisulphate.  I have seen this in a number of methods. Does anyone have an explanation for this? ie. Is that temperature really that important? Thankyou.
Angela Hammond
Histology Dept.
Clinpath Laboratories
Adelaide, South Australia

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