RE: Possibility of to reuse histochemical reagents

From:Tony Henwood

RE: Possibility of to reuse histochemical reagents

Lina,
Answers as follows:
-----Original Message-----

I have a question about the histochemicals methods. For make the
histochemical study of lipids and carbohydrate and proteins, can I reuse the
reagents in the next methodos:

PAS - Periodic acid (1%) up to a week, Schiff's - until it discolours
Alcian Blue pH (0.5,1.0 and 2.5)- Dependant on useage- 1 month

Bromophenol Blue

Red O - 1-2 hours
Sudan Black
Aqueous Blue Nile
Ferric Haematoxylin - 1 hour

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html





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