Zory

Did you inject the animals with BrdU about 1 to 2 hours prior to tissue
collection?  I'm not familiar with the amersham kit, but I have used the
amersham anti-BrdU mouse monoclonal antibody. I use this antibody on rat
tissue.  If I'm doing BrdU on mouse I prefer the rat monoclonal BrdU
from Accurate.  I have procedures for both of these antibodies if you
would like them.  


Elizabeth A. Chlipala, BS, HTL(ASCP)
Premier Histology Laboratory, LLC
P.O. Box 18592 
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
lizchlipala@premierhistology.com
www.premierhistology.com
 
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-----Original Message-----
From: Shaposhnik, Zory [mailto:ZShaposhnik@mednet.ucla.edu] 
Sent: Wednesday, April 23, 2003 4:04 PM
To: 'histonet@pathology.swmed.edu'
Subject: In vivo proliferation in vascular sections using Amersham BrdU
ki t

 Hello,

I have tried to use the Amersham BrdU kit to look at proliferation in
frozen
mouse aortic sections and have not seen any staining with DAB. 5uM
spleen
sections were used as a positive control. No staining was seen there
either.
I confirmed that the Peroxidase linked secondary anti-mouse Ig 2a
antibody
works.

Does anyone have any suggestions? Is a PCNA stain an easier way to look
at
proliferating cells in frozen sections? If so, what kit/technique would
be
best?

Thank you,

Zory Shaposhnik
Division of Cardiology
UCLA