We have been staining IL-1a in the past on acetone-fixed cryostat sections
using a regular 3-step streptavidin-biotin-based detection system. In our
atherosclerotic specimens we found a IL-1a membrane staining of macrophages
and sometimes smooth muscle cells. At that time we were using a rabbit
anti-IL-1a antibody from Genzyme, unfortunately not available anymore.
You are lucky that IL-1a is a membrane bound cytokine and does not leak
away during fixation or later washing steps like other cytokines as IL-2,
IL-4, IL-6, IFNgamma.
Furthermore, be aware that anti-cytokine antibodies are prone to stain a
plasma cell subpopulation non-specifically. See: JHC 2001 46:699-709 and
handout Int. lecture NSH 2001.
Chris van der Loos
Academical Medical Center
Dept. of Cardiovascular Pathology
Amsterdam - The Netherlands
Sally Ann Drew wrote:
>Date: 23 Apr 2003 13:31:05 -0600
>From: "Drew Sally A."
>Is there anyone around with professional, calm, non-hackle raising words of
>wisdom concerning the performance of IL-1a in IHC? (:)]
>Sally Ann Drew-MT(ASCP)
>Univ. of Wisconsin Hospital & Clinics
>600 Highland Ave. VAH-DM223
>Madison, WI 53792-2472
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