RE: IL-1a

From:Chris van der Loos

Dear Sally,
We have been staining IL-1a in the past on acetone-fixed cryostat sections 
using a regular 3-step streptavidin-biotin-based detection system. In our 
atherosclerotic specimens we found a IL-1a membrane staining of macrophages 
and sometimes smooth muscle cells. At that time we were using a rabbit 
anti-IL-1a antibody from Genzyme, unfortunately not available anymore.
You are lucky that IL-1a is a membrane bound cytokine and does not leak 
away during fixation or later washing steps like other cytokines as IL-2, 
IL-4, IL-6, IFNgamma.
Furthermore, be aware that anti-cytokine antibodies are prone to stain a 
plasma cell subpopulation non-specifically. See: JHC 2001 46:699-709 and 
handout Int. lecture NSH 2001.
Good luck!

Chris van der Loos
Academical Medical Center
Dept. of Cardiovascular Pathology
Amsterdam - The Netherlands

Sally Ann Drew wrote:
 >Date: 23 Apr 2003 13:31:05 -0600
 >From: "Drew Sally A." 
 >Subject: IL-1a
 >Is there anyone around with professional, calm, non-hackle raising words of
 >wisdom concerning the performance of IL-1a in IHC? (:)]
 >Sally Ann Drew-MT(ASCP)
 >Univ. of Wisconsin Hospital & Clinics
 >IHC/ISH Laboratory
 >600 Highland Ave. VAH-DM223
 >Madison, WI 53792-2472

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