From:Tony Henwood

A simple stain to use:

Document Procedure:

Mallory-Parker's Haematoxylin For Copper And Lead



This technique is based on the ability of unripened haematein to form a coloured dye-lake with copper and Lead. It is very simple to perform and usually produces good results.

Fixation: 10% buffered formalin.

Microtomy: paraffin sections at 5um.

Controls: Liver from Wilson’s disease or Primary Biliary Cirrhosis


  1. Haematoxylin Solution

Dissolve 0.1g Haematoxylin (CI 75290) in a few drops of absolute alcohol; mix with 10ml of 2% dipotassium hydrogen phosphate.


1. Dewax and hydrate sections.

2. Stain in haematoxylin solution 1 1/2 - 2 hours, at room temp.

3. Wash in running water 10 minutes.

4. Dehydrate, clear and mount.


Copper blue

Lead dark blue-grey


Culling, C.F.A, (1974) "Handbook of Histopathological and Histochemical Techniques" 3rd ed, Butterworths, London p388-389.

-----Original Message-----
From: Nejat Yilmaz []
Sent: Tuesday, 15 April 2003 18:05

Hi histonetters,
 I found a stain for lead, Mallory-Parker's method. But unfortunately I could not find its protocol...Does anybody have experience about this stain and its protocol ?
Thanks in advance..

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