| From: | Tony Henwood |
Document Procedure:
Mallory-Parker's Haematoxylin For Copper And Lead
Principle:
This technique is based on the ability of unripened haematein to form a coloured dye-lake with copper and Lead. It is very simple to perform and usually produces good results.
Fixation:
10% buffered formalin.Microtomy
: paraffin sections at 5um.Controls:
Liver from Wilson’s disease or Primary Biliary CirrhosisReagents:
Dissolve 0.1g Haematoxylin (CI 75290) in a few drops of absolute alcohol; mix with 10ml of 2% dipotassium hydrogen phosphate.
Procedure:
1. Dewax and hydrate sections.
2. Stain in haematoxylin solution 1 1/2 - 2 hours, at room temp.
3. Wash in running water 10 minutes.
4. Dehydrate, clear and mount.
Results:
Copper blue
Lead dark blue-grey
Reference:
Culling, C.F.A, (1974) "Handbook of Histopathological and Histochemical Techniques" 3rd ed, Butterworths, London p388-389.
-----Original Message-----
From: Nejat Yilmaz [mailto:nyilmaz@mersin.edu.tr]
Sent: Tuesday, 15 April 2003 18:05
To: histonet@pathology.swmed.edu
Subject:Hi histonetters,I found a stain for lead, Mallory-Parker's method. But unfortunately I could not find its protocol...Does anybody have experience about this stain and its protocol ?Thanks in advance..
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